B iological R esearch i n C anisters BRIC-LED

1. Biological Research in CanistersBRIC-LED Actuation tool interface • Up to six 60mm x 15mm Petri Dishes per BRIC canister • Eight canisters per half locker tray • 3 sealed levels of containment • Unidirectional LED illumination • Single or dual stage manual liquid (fixatives, growth inhibitors, etc.) introduction • Autonomous environmental data logging Illumination controls BRIC-LED Canister Petri Dish Sealed Petri Dish Fixation Unit Liquid reservoir Enables Liquid Treatments (e.g. Cytochalasin) Followed by In-flight Fixation ESC 00415—EAR 99

2. Biological Research in CanistersBRIC-Opti Gas sampling port • 2 sealed levels of containment • Gas sampling capable • Passive • Can be frozen (-100 °C) • Contains up to 4 OptiCell™ culture chambers • Autonomous environmental data logging Lid OptiCell™ Culture Chambers Multichannel temperature and humidity logger Base OptiCell™ Culture Chambers For Passive Tissue Culture/Microbial Growth in COTS Opticells ESC 00415—EAR 99

3. Kennedy Space Center Fixation Tube(KFT) Main Tube Expansion Plug • Provides three levels of containment during all phases • No glovebox required • Samples may be transferred from other growth facilities • Maintains robust containment down to -100 °C Actuator Sample Tube 2.365” L x 0.875” D • Chemical Preservative: • 3% glutaraldehyde • 5% formaldehyde • 5% DMSO • RNAlater™ • b-glucuronidase stain • 5% formalin • 5% acetic acid • 63% ethanol • 0.5% glutaraldehyde • 2% paraformaldehyde ESC 00415—EAR 99 For Crew-Facilitated In-Flight Specimen Fixation

4. FASTRACK™ Suborbital Platform • Provides compatibility across the entire fleet of emerging suborbital vehicles, existing and future parabolic flight providers, and future orbital free-flyers or transports • Supports 2 single middeck locker equivalents or 1 double locker • Provides power and data acquisition FASTRACK Structural View Parabolic Flight Testing Provides a Standard Platform for Suborbital and Parabolic Investigations ESC 00415—EAR 99

5. Payload Integration & Operations • 52 payloads have been managed and integrated • Platforms: shuttle, ISS, SPACEHAB, Progress, MPLM, Spacelab, Mir • Joint foreign collaborations with CSA, DLR, ESA, JAXA, NSAU • Capabilities for hardware engineering, integration, operations support, and project science coordination • Experiment Monitoring Area (EMA) operations control center at KSC for real time support • ISS Environmental Simulator (ISSES) chambers provide simulated spaceflight environment for analog ground controls ESC 00415—EAR 99 Integration History and Capabilities

6. Pre-Phase A Phase A Phase B Phase C Phase D Phase E Definition Operations & Analysis Solicitation & Selection Integration Verification Development Payload Development:A Phased Approach • Each phase includes a final milestone or gate • Each phase has distinct activities associated with science, hardware, and analytical integration • Phase duration is a function of many factors • Experiment maturity • Hardware maturity • Platform ESC 00415—EAR 99 Payload Lifecycle

7. Payloads in the land of “OZ” Payload Integration Manager Schedule STRATEGIC TACTICAL PDR CDR CoFR SafetyPhase II Payload Pre-Ship Safety Phase 0/I Payload Manifested Safety Phase III • Safety Data Pack • Export Classification Letter • Prelim Design Data Package • Safety Data (Phase 0-I) • Training Strategy TIMs • C&DH DS (P) • KSC Support Rqmts DS • KSC Technical Rqmts DS • Ground Data Services DS • Procedures & Displays (U) • Payload Planning DS (P) • Manifest/Stowage DS (P) • Drawing DS (P) • Manifest/Stowage DS (U) • Drawing DS (U) • Integration Data Pack (IDP) • Payload Integration Agreement (PIA) • C&DH DS (F) • Procedures & Displays (F) • Critical Design Data Package • Safety Data (Phase II) • ICD/Verification Requirements • C&DH DS (U) • Payload Trainer H/W & S/W • Training Plans & Courseware • Payload Planning DS (F) • Resource Requirements Definition • Procedures & Displays DS (P) • Interface Control Document (ICD) (U) • Payload Verification Plan (PVP) (U) • Requirements Exceptions Key: P – Preliminary U – Update F – Final • CoFR Endorsement ESC 00415—EAR 99 Why an Implementation Partner is Important

8. Acronyms ABRS Advanced Biological Research System BRIC Biological Research in Canisters BRIC-LED Biological Research in Canisters – Light Emitting Diode COTS Commercial Off-the-Shelf CSA Canadian Space Agency DLR DeutschesZentrumfürLuft- und Raumfahrt DMSO Dimethyl sulfoxide ECP Environmental Control Panel EMA Experiment Monitoring Area ERC Environmental Research Chamber ESA European Space Agency EXPRESS Expediting the Processing of Experiments to the Space Station GCU Generic Containment Unit GFP Green Fluorescent Protein ISS International Space Station ISSES ISS Environmental Simulator JAXA Japan Aerospace Exploration Agency KFT Kennedy Space Center Fixation Tube KSC Kennedy Space Center LSSC Life Science Services Contract MPLM Mult-purpose Logistics Module NSAU National Space Agency of Ukraine TOSC Test and Operations Support Contract ESC 00415—EAR 99

9. Points of Contact Ground Research Support Point of Contact (ESC): Michele N. Birmele Advanced Life Support Laboratories Lead Mail Code ESC-24 Kennedy Space Center, FL 32899 Phone 321.861.2940 Michele.N.Birmele@nasa.gov Laboratory Support Point of Contact (TOSC): Bruce E. Burch TOSC Project Manager Mail Code 721S-S100 Kennedy Space Center, FL 32899 Phone 321.867.5255 Bruce.E.Burch-1@nasa.gov Hardware + Implementation Point of Contact (ESC): David W. Reed Payload Engineering Lead Mail Code ESC-24 Kennedy Space Center, FL 32899 Phone 321.861.2980 David.W.Reed@nasa.gov ESC 00415—EAR 99

10. Thank you! ESC 00415—EAR 99