DNA vs RNA

1. DNA vs RNA Both are nucleic acids… Be able to compare these two nucleic acids

2. DNA Monomer: Nucleotide • 1 phosphate group • 1 deoxyribose 5-carbon sugar • 1 nitrogen-containing base (A, C, G, or T)

3. DNA polynucleotide • Alternating sugar and phosphate molecules form backbone • Nitrogenous bases form complementary pairs joined by hydrogen bonds • Adenine (A) pairs with Thymine (T) • Cytosine (C) pairs with Guanine (G)

4. DNA Bases

5. RNANucleotides • 1 phosphate group • 1 ribose sugar • 1 nitrogenous base (A, C, G, or U) • Uracil replaces thymine in RNA

6. Protein Synthesis

8. InformationFlow • Genetic information in DNA is transcribed to RNA (transcription), then translated to protein (translation) • The molecular basis of phenotypic traits are proteins that serve many different functions

9. Transcription & Translation of RNA Codons

10. One gene - One protein ? • Each protein made at the ribosomes is controlled by a specific DNA gene (based on sequence of DNA base pairs) • Changes in DNA base sequence can ultimately result in changes in the protein produced in cell

11. RNA Transcription • DNA nucleotides are transcribed into RNA nucleotides in nucleus • Adenine pairs with Uracil • Cytosine pairs with Guanine • Every 3 bases in DNA make 1 RNA triplet codon

12. RNA transcription • RNA polymerase allows DNA nucleotides to be transcribed into RNA nucleotides and then links them together • 3 different types of RNA are transcribed: • Messenger RNA (mRNA) • Transfer RNA (tRNA) • Ribosomal RNA (rRNA)

13. The Genetic Code • 1961: The first RNA triplet codon was decoded and found to translate into a specific amino acid • The genetic code is basically the same for all organisms, so bacterial cells can translate genetic messages from humans, and vice versa. • Useful for Recombinant DNA technology

14. Protein Synthesis

15. Transcription 3’ 5’

17. Transcription of a Gene

19. primary mRNA processing At the ends of each intron are Small nuclear ribonucleoproteins or snRNP’s And these recognize splice sites Many snRNP’s join with protein = splicesome Ribozymes = RNA’s that function as enzymes Some intron RNA’s function as ribozymes to catalyze their own excision

20. Translation

21. Translation involves complex biochemistry! …Correct matches between tRNA and aa (aminoacyl-tRNA synthetase) …Correct match between tRNA anticodon and the mRNA codon …Ribosomes facilitate the coupling of tRNA anticodons with mRNA codons during protein synthesis …rRNA has three binding sites for tRNA …….A site= holds the tRNA carrying the next amino acid to be added to the growing polypeptide …….P site = holds the tRNA carrying the growing poly- peptide chain …….E site = exiting tRNA

22. Transfer RNA

23. Transfer RNA • Transfer RNA serve as “interpreters” during translation • Each has an anticodon region that binds to specific codons on the messenger RNA • Each also has an attachment site for an amino acid

24. E P A 3’ 5’ mRNA 3’ 5’

25. Initiation – mRNA, tRNA, aa 2 rRNA subunits Ribosome scans downstream 5’ 3’ Elongation – aa are added one by one to the preceding aa mRNA is moved thru the ribosome in one direction only..5’ end first Ribosome and mRNA move relative to each other codon by codon Termination – stop codon reaches the A site of ribosome UAG, UAA, or UGA E P A U A C 3’ A U G 5’

26. E A P

27. E P E P A E P A E P A

28. Summary of transcription and translation E E E

29. Flow of genetic information • DNA to RNA to protein • Polyribosomes = many ribosomes read one mRNA • Post-transcriptional Modificaiton • Coil and folding polypeptide • Modify aa by adding a sugar, lipid or phosphate group • Enzymes may remove one or more aa • Enzymes may cut protein in ½ • Free ribosomes = proteins function and dissolve in cytosol • Bound ribosomes = ER or Nuclear Membrane bound endomembrane system

30. Gene Expression RNA has many roles in a cell Point Mutations Substitutions Insertions/Deletions Mutagens What is a gene?

31. Sickle cell anemia: a single base mutation