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Care and Use of the Microscope

Care and Use of the Microscope. Replace dust cover and put up after use Hold with one hand on the arm and one on the base Only use lens paper to clean microscope lens Report any problems to me. Let the cord hang off the lab table Drag the microscope across the lab table

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Care and Use of the Microscope

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  1. Care and Use of the Microscope

  2. Replace dust cover and put up after use Hold with one hand on the arm and one on the base Only use lens paper to clean microscope lens Report any problems to me Let the cord hang off the lab table Drag the microscope across the lab table Try to disassemble the microscope Do’s and Don’ts

  3. Calculating Magnification • Two lenses • Objective lens • Ocular lens (eyepiece) • Objective lens • Usually 3-4 • Common set: scanning power (4x), low-power (10x), high-power (40x), and oil immersion (100x) • Ocular lens • (10x) • Total Magnification= objective lens magnification x ocular lens magnification

  4. Example Problem 1 Q: If the ocular lens has a power of 10x and the objective lens has a power of 100x, what is the total magnification?

  5. Example Problem 1 Q: If the ocular lens has a power of 10x and the objective lens has a power of 100x, what is the total magnification? A: Multiply the ocular power times the objective power 10x * 100x=1000x Total Magnification=1000x

  6. Example Problem 2 Q: If the ocular lens has a power of 5x and the objective lens has a power of 40x, what is the total magnification?

  7. Example Problem 2 Q: If the ocular lens has a power of 5x and the objective lens has a power of 40x, what is the total magnification? A: Multiply the ocular power times the objective power 5x * 40x=200x Total Magnification=200x

  8. Preparing a slide • ((Didn’t use this in class this time, but I figured I’d leave it in)) • To make a temporary wet mount slide • Get a clean slide • Place a tiny, thin piece of the specimen on the slide • Place a small drop of water over the specimen • Place a cover slip over the specimen • For oil emersion put a drop of oil on top of the cover slip

  9. For this Lab • Not making a wet mount • Remember when looking into the microscope to try adjusting the following to get a good view…. • Coarse focus • Fine focus • Iris Diaphragm • Position of the slide • Level of magnification

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