Andor Revolution Spinning Disk System – Mosaic Manual

1. Andor Revolution Spinning Disk System – Mosaic Manual

2. System Overview Mosaic Targeted Illumination Yokogawa Spinning Disk

3. Mosaic – Targeted Illumination –Overview

4. Alignment Reasoning • Calibration is required to align Mosaic micromirrorsto CCD pixels • Calibration should be performed before each session, for each required objective

5. Alignment Procedures 4.2 4.3 4.7 4.7 4.7 4.8 1 2 4.4 4.1 4.4 4.6 4.0 4.4 4.4

6. View Overlay Area

7. Create Targeted Illumination ROIs

8. Targeted Illumination Taskbar Functions Opens the Mosaic Targeted Illumination window Opens the Multi Dimensional Acquisition window Saves region masks using the save name assigned in the multi dimensional acquisition window. Before activating this function, verify that a unique save name has been assigned in the MDA window and verify that the folders in the save path exists. The maximum exposure length of the targeted illumination is 1000000ms (.27hours). This value is assigned to a variable but is not applied until the regions are actually pulsed. This journal is used during the MDA, it loads the regions and pulses the sample. This is a simple pulse of the latest active mask. 1 2 3 4 5 6

9. Suggested Workflow Using MDA & Mosaic