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Relative Luciferase activity

TSP1. CT. VWC. IGFBP. C. N. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. *. AV E G D NCVF D GVIYRSG E KFQ P SCKFQCTCR D GQIGCV P RCQL D VLL P E P NC P A P RKV E V P G E CC E KWICG P DEED SLGGLTL. 14. +/-. -. +++. 12. 10.

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Relative Luciferase activity

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  1. TSP1 CT VWC IGFBP C N * * * * * * * * * * * * * * * * * * * * * * AVEGDNCVFDGVIYRSGEKFQPSCKFQCTCRDGQIGCVPRCQLDVLLPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTL 14 +/- - +++ 12 10 Time of incubation (hours) b-Galactosidase activity in yeast 8 Gal4 DBD-NH2 - - - - 0,5 1 2 4 6 Gal4 DBD-NH3 - - +/- - Gal4 DBD-NH4 Gal4 DBD-NH5 - - - - - - - - 4 2 Gal4 DBD-NH23 - + ++ +++ 0 Gal4 DBD-NH24 + ++ +++ +++ Gal4 DBD-NH25 - - - - Gal4 DBD-NH35 - - - - Gal4 DBD-NH45 - - - - 6HIS NH3 Gal4 DBD Luciferase 5 Gal4 DBD binding sequences in tandem TATA mini Promoter 12,90 Gal4DBD-NH3 9,77 Relative Luciferase activity 2,11 1,00 Immunocytodetection 1ug 100ng 500ng 1ug pFA NH3 NH2 NH3 NH4 NH5 A) CCN3 B) C) BHK21 cells

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