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luciferase cells

Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Learn more from https://www.creative-biogene.com/genesearch/Luciferase.html

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luciferase cells

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  1. ApplicationofLuciferaseCells WhatisLuciferaseCells? LuciferaseCell(Englishname:Luciferase)isthegeneralnameoftheenzymesthatcanproduce bioluminescenceinnature.ThemostrepresentativeoneisLuciferaseinfirefly,whichiscalled Photinuspyrali.Fluorescenceisproducedbytheoxidationofluciferininthecorresponding chemicalreactions,andinsomecasesthereactionsystemalsoincludesadenosinetriphosphate (ATP).Withoutluciferase,luciferinreactsveryslowlywithoxygen,andthepresenceofcalcium ionsoftenacceleratesthereactionfurther(similartomusclecontraction). Luciferinorluciferaseisnotaspecificmolecule,butratherageneraltermforallthesubstrates andcorrespondingenzymesthatproducefluorescence,althoughtheyvary.Differentluminescent organismsusedifferentluciferaseenzymestocatalyzedifferentluminescentreactions.Thebest knownbioluminescentorganismisthefirefly,whichUSESdifferentluciferaseenzymesfromother bioluminescentorganismssuchasthefireflymushroom(Omphalotusoleariu')ormanyMarine organisms.Infireflies,theoxygenneededforthebioluminescentresponseispumpedintoatube calledtheabdominaltrachea.Someorganisms,suchastheelater,containavarietyofdifferent

  2. luciferaseenzymesthatcatalyzetheproductionofdifferentcoloursoffluorescencefromtheluciferaseenzymesthatcatalyzetheproductionofdifferentcoloursoffluorescencefromthe samesubstrate.Therearemorethan2,000speciesoffireflyandmanymoreintheelateroidea (includingfireflies,elateritesandrelatedinsects),sotheirluciferaseisusefulformolecularphylo ApplicationofLuciferaseCells Luciferasecanbeproducedinthelaboratoryusinggeneticallyengineeredmethodsandisusedin avarietyofexperiments.Luciferasegenescanbesynthesizedandinsertedintolivingorganisms ortransfectedintocells.Researchershaveusedgeneticengineeringtomakeluciferaseinmice, silkworms,potatoesandotherorganisms.Indirectlyinvitroimagingisapowerfulmeansof research,toanalyzethewholeanimalbodycellsinthecommunity:differenttypesofcells(bone marrowstemcellsandTcells,etc.)tag(expression)luciferinenzyme,canusethehighsensitivity oftheCCDcameratoliveanimalstoobserveanddonotharmtotheanimalsthemselves. Fluorescencecanbeemittedbyaddingthecorrectluciferinsubstratetoluciferase,andthe emittedphotoncanbedetectedbylightsensitiveelementssuchasfluorescencedetectoror improvedopticalmicroscope.Thismakesitpossibletoobservethecourseofmanylifeprocesses, includinginfection.Forexample,luciferasecanbeusedtodetectwhetherredbloodcellsinblood Banksarebeginningtobreakdown.Forensicscientistscanuseasolutioncontainingluciferaseto detecttracesofbloodleftatacrimescene.Hospitalsuseluciferaselighttodetectspecific diseases.Luciferasecanalsobeusedasa"reporterprotein"inmolecularbiologystudies,for example,todetecttranscriptionofspecificpromotersincellstransfectedwithluciferaseorto detectintracellularATPlevels.ThetechniqueiscalledreporterAssayorLuciferaseAssay. Luciferaseisaheatsensitiveproteinandisoftenusedtostudytheprotectivecapacityofheat shockproteinsduringproteinthermaldegeneration.

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