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LUCIFERASE

LUCIFERASE. A. Cengiz Öztireli. BIOLUMINESCENCE. BIOLUMINESCENCE (LIVING LIGHT) Milky Sea. BIOLUMINESCENCE. Applications. BIOLUMINESCENCE. B acteria. S ea A nemones. D inoflagellates. BIOLUMINESCENCE. FIREFLYES (PHOTINUS) Attracting mates Attracting prey. LUCIFERASE. FIREFLY.

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LUCIFERASE

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  1. LUCIFERASE A. Cengiz Öztireli

  2. BIOLUMINESCENCE • BIOLUMINESCENCE (LIVING LIGHT) • Milky Sea

  3. BIOLUMINESCENCE • Applications

  4. BIOLUMINESCENCE Bacteria Sea Anemones Dinoflagellates

  5. BIOLUMINESCENCE • FIREFLYES (PHOTINUS) • Attracting mates • Attracting prey

  6. LUCIFERASE • FIREFLY • BACTERIA

  7. Luciferase + O2 Luciferin Inter. P* P + hv BIOLUMINESCENCE • 8 ATP → 1 Green Photon • Reaction: ATP

  8. BIOLUMINESCENCE • Color • P* • P* + F → F* + P • Proteins around • Structure

  9. PHOTINUS PYRALIS (FIREFLY)

  10. PHOTINUS PYRALIS LUCIFERASE Luciferase + Luc-COOH ATP Luciferase: Luc-CO-AMP O2 Luciferase: Luc=O*

  11. PHOTINUS PYRALIS LUCIFERASE • Structure and Functioning C-Terminal Cleft N-Terminal

  12. CONCLUSIONS • THANK YOU

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