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HPTS- Optical pH Sensor

pH sensitive fluorescent dye Absorbance / Excitation changes with pH Emission at 520 nm Excitation Ratiometric. HPTS- Optical pH Sensor. pH SENSOR ASSEMBLY FOR OPTICAL CHEMICAL SENSOR. White microfiltration membrane backing. PEG-dye copolymer hydrogel. Transfer adhesive.

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HPTS- Optical pH Sensor

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  1. pH sensitive fluorescent dye Absorbance / Excitation changes with pH Emission at 520 nm Excitation Ratiometric HPTS- Optical pH Sensor

  2. pH SENSOR ASSEMBLY FOROPTICAL CHEMICAL SENSOR White microfiltration membrane backing PEG-dye copolymer hydrogel Transfer adhesive Note: All Optical Chemical Sensors Are Based on Same Concept as pH Sensor

  3. pH Sensor Calibration in Cell Suspension

  4. E.coli Shake Flask Fermentation with pH Coaster and Sensor Patch • Conditions: LB Media, T=37°C, 300 rpm • Discrepancy at 6 hrs+ likely due to CO2

  5. Measurement of pCO2 • Important metabolite • Critical in bacterial and mammalian cell cultures • Conventional technology uses pH probe inside bicarbonate buffer- cumbersome • New optical alternative

  6. APPLICATION OF OPTICAL CHEMICAL CO2 SENSOR IN FERMENTATION: E. COLI CO2 OD

  7. DISSOLVED OXYGEN OPTICAL-CHEMICAL SENSOR CONSTRUCTION

  8. On-line turbidity measurement in E.coli fermentation

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