1 / 23

iGEM @ Imperial

iGEM @ Imperial. Week 1 Engineering/Biology Introduction Lectures Journal Club Wiki Brainstorming 3 ideas. Starting off: Week 1. Only three ideas made the cut. Bio-Clock (Re-defining Time). Pulse of AHL moves along a gutter of medium Cells fluoresce when activated Refractory period

kermit-baxter
Download Presentation

iGEM @ Imperial

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. iGEM @ Imperial

  2. Week 1 Engineering/Biology Introduction Lectures Journal Club Wiki Brainstorming 3 ideas Starting off: Week 1

  3. Only three ideas made the cut.

  4. Bio-Clock (Re-defining Time) • Pulse of AHL moves along a gutter of medium • Cells fluoresce when activated • Refractory period • The time period is controlled by • the radius • gutter width • cell density

  5. Bio-Memory 1010100000000011111000010101011111100000000111111111000001010100010100001000010100101001010000001001010101001010101001101010101001010 • Data stored in bacteria written using a green laser • Data read using a UV laser + fluorimeter • Cells either 1 (RFP) or 0 (no RFP) • Data stored in switch Very High Compression due to small size of bacteria

  6. The Oscillator • Culture Wide oscillations of AHL • Frequency must be tuned easily • Oscillations must be stable Changing Concentration of AHL

  7. Week 2 Investigation of all 3 ideas Modelling Evaluating risks Start work in the Wet lab Decision on the Oscillator as main project;can use other ideas as further developments Week 3 Further research Modelling Assembly of parts Protocols for testing parts Setting up OWW

  8. Predator Prey Dynamics Simply Make a Bio-chemical system that can do this. Lotka-Voltarra Model output

  9. A A B B Design • Positive Feedback of A • AB Induces production of more B • Both A and B are used to make AB

  10. A B Two Cell System • Two independent populations of Cells • These cells do not kill each other • Altering the initial ratios of these cells will alter the frequency of oscillations

  11. A A A A Design Cell1 (Prey) • Prey cell must produce molecule A exponentially Lux R is produced which detects molecule A Pc Lux R Then initiates transcription at Plux Pc is always on Lux I Plux Which Produces More A

  12. Design The Predator Cell The role of a predator is to reduce the prey numbers as a function of the predator population numbers. Predator Detect Prey Population Size Reduce Prey Population Size

  13. Lux R aiiA Plux Design The Predator Cell Detects Prey Population Size Reduces Prey Population Size

  14. Design The Predator Cell A LuxR A A LuxR aiiA Lux R aiiA Plux

  15. Pc Lux R Lux I Plux A A A Design (Entire System) Diffusion Extra cellular pool of A (HSL) (this should oscillate) The Prey Cell The Predator Cell LuxR A A LuxR aiiA Lux R aiiA Plux

  16. Modelling • Tom’s Monster

  17. Modelling

  18. Testing parts: T9002

  19. A shocking discovery: at first sight... • After finishing our oscillator design... • MIT Project 2004: • Cell-Cell synchronized Oscillator Design • Similar approach using concepts of quorum sensing • BUT: This system does not use predator-prey dynamics and is implemented in a single cell (ours is multicellular MIT Oscillator Design http://web.mit.edu/~cbatten/www/work/ssbc04/system-spec-ssbc04.pdf

  20. Communication: The Wiki • Wiki-Newspaper • Documentation for future references • Communication • Within the team • With other teams • Monitoring progress (Gantt Chart) • Present ourselves & our project

  21. Outline • Further modelling & testing of parts • Parts assembly • Phase 2 • Coupling the oscillator to a biological to electrical interface • Synchronizing oscillations 2 petri dishes

  22. Thank you

More Related