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DietGel Study: Impact of 76A and Boost on the Performance on C57BL/6J Timed-Pregnant Females

DietGel Study: Impact of 76A and Boost on the Performance on C57BL/6J Timed-Pregnant Females. Tufts University and ClearH2O. Study Design Details. 5 groups of timed pregnant females Regular Chow only Regular Chow + 76A gel Regular Chow + Boost gel Breeder Chow only

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DietGel Study: Impact of 76A and Boost on the Performance on C57BL/6J Timed-Pregnant Females

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  1. DietGel Study: Impact of 76A and Boost on the Performance on C57BL/6J Timed-Pregnant Females Tufts University and ClearH2O

  2. Study Design Details • 5 groups of timed pregnant females • Regular Chow only • Regular Chow + 76A gel • Regular Chow + Boost gel • Breeder Chow only • Breeder Chow + Boost Gel • Cage Manipulation • Cages were changed every Monday by Rodent Breeding Service (RBS) staff • Cages were checked and gel added on Wednesday and Friday every week • Each cage was taken off of the shelf, opened, and hand put in the cage whether gel was to be added or not so that each cage was ‘disturbed’ 3x per week including cages that didn’t need gel to avoid variability. • Pup Information • Pup/litter deaths were recorded at cage change, health monitoring check or gel replacement check • Pups were weighed on P7, P14, P21, and P28 individually and weights were totaled for litter weight and averaged for average pup weight in each litter

  3. Pup Loss

  4. Feb 22 Photos Regular Chow Female Regular Chow + 76A Female Boost Female

  5. March 5 (P14) Photos Regular Chow + 76A Pups P14 Regular Chow Pups P14

  6. March 12 (P21)Photos Regular Chow + 76A Pups P21 Regular Chow Pups P21

  7. Breeder Chow Pups P21

  8. Average Pup Weights Over The Course of the Study

  9. Conclusion • 76A supplemented group demonstrated significantly less pup loss • 3% pup loss • No lost litters • 76A supplemented group demonstrated greater average pup weights – up to 20% heavier than controls • Recommend using 76A as a supplement for pregnant females or breeders to improve pup yield, and to improve weight gain for fragile strains

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