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Types of Microscopes

Types of Microscopes. Unit 3 – Powerpoint #1 Honors Biology 2012-2013. Light Microscopes. Use a beam of Light The specimen can be dead or alive. Simple Microscopes. Have only 1 lens Give two examples of a simple microscope:. Magnifying Glass Reading Glasses. Simple Microscope.

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Types of Microscopes

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  1. Types of Microscopes Unit 3 – Powerpoint #1 Honors Biology 2012-2013

  2. Light Microscopes • Use a beam of Light • The specimen can be dead or alive

  3. Simple Microscopes • Have only 1 lens • Give two examples of a simple microscope: • Magnifying Glass • Reading Glasses

  4. Simple Microscope

  5. Compound Microscopes lens • Have more than one ______

  6. Dissecting Microscopes 20x 30x • Magnify by 10x or ______ or ________ • Shine light on or ____________ the specimen • Specimen can be thick or _______. through thin

  7. Dissecting Microscope

  8. Light Microscope 40- 400x • Magnify by ______________ • Shines light ______________ the specimen • Specimen must be _________. through thin

  9. Light Microscope Image

  10. Electron Microscope electrons dead • Use a beam of _______________- • The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________ air water

  11. Scanning E.M. • Makes 3D pictures of surfaces • Magnify by _________ 60,000x

  12. Scanning E.M. Image (3D)

  13. Transmission E.M. 2D • Makes _____ pictures of thin slices • Magnify by __________ 100,000x

  14. Transmission E.M. Image (2D) Butterfly Antenna

  15. Other Microscopes • Probe Microscopes • Ion Microscopes • _____________ can be seen with these 2 types of microscopes. Atoms

  16. Probe Microscope

  17. Probe Microscope Image • Horse Hair

  18. Ion Microscopes

  19. Ion Microscope Images

  20. Microscope Parts and Functions

  21. 1. Eyepiece Magnifies by 10x

  22. 2. Revolving Nose Piece Changes Objective Lens

  23. 3. Low power Objective Lens Magnifies by 4x (Red)

  24. 4. Medium Power Objective Lens Magnifies by 10x (yellow)

  25. 5. High Power Objective Lens Magnifies by 40x (Blue)

  26. 6. Stage Clips To hold slide in place

  27. 7. Diaphragm Adjusts the amount of light

  28. 8. Light Light to see specimen

  29. 9. On/Off Switch Turns light on and off

  30. 10. Arm To hold while carrying

  31. 11. Stage To set slide on

  32. 12. Course Adjustment To focus only when in Low Power

  33. 13. Fine Adjustment To focus when using medium or high power

  34. 14. Base • To hold while carrying

  35. Both Arm A. Carry a microscope with ___________ hands – one hand holding the _______ And the other hand under the ___________ Always hold the microscope upright so the _____________ cannot fall out When you place a microscope at your table, make sure it is _______ from the edge If there is anything wrong with the microscope, or it is damaged in any way, let the _______________ know immediately. B. To clean the objective lenses and eyepiece, use _______________________ Base Eyepiece Away Teacher Lens Tissue

  36. Prepare your slide • Plug the microscope in. Turn on the ______________ • Be sure the ___________ Power Objective Lens (_______) is clicked into place. • NOTE: Be sure to look at the microscope from the side when changing objective • lenses to avoid damaging the ________________ and _____________ • 4. Use coarse focus to raise the stage to its ________________ position • 5. Place the _______________ on the stage. • Look through the eyepiece and turn the __________________ (lowering the stage) • until the specimen is visible. • 7. Use the ____________ adjustment to bring your specimen into sharp focus. • You may wish to move the ________________ to adjust the amount of light. Light Low 4X Slide Lens Highest Slide Coarse Focus Fine Diaphragm

  37. Focused 8. Make sure the specimen is ______________ 9. Switch to the ___________ Power Objective Lens ( ______) NOTE: Look at the microscope from the ____________ when you change lenses. 10. To avoid losing your focus and/or crushing the slide with the lens, never use the __________________ focus on medium or high power. Use the _____________ focus only to make the specimen visible. 11. Make sure the specimen is ______________. Look from the _________ as you change objective lenses and switch to the __________ Power Lens (________) 12. Use only the _____________ focus to make the specimen visible. 13. When finished, make sure the ___________ power objective lens is in place, wrap the __________ around the _________ of the microscope, cover, and place in the appropriate location Mid 10X Side Coarse Fine Focused Side High 40X Fine Low Cord Arm

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