Types of Microscopes

1. Types of Microscopes Unit 3 – Powerpoint #1 Honors Biology 2012-2013

2. Light Microscopes • Use a beam of Light • The specimen can be dead or alive

3. Simple Microscopes • Have only 1 lens • Give two examples of a simple microscope: • Magnifying Glass • Reading Glasses

4. Simple Microscope

5. Compound Microscopes lens • Have more than one ______

6. Dissecting Microscopes 20x 30x • Magnify by 10x or ______ or ________ • Shine light on or ____________ the specimen • Specimen can be thick or _______. through thin

7. Dissecting Microscope

9. Light Microscope 40- 400x • Magnify by ______________ • Shines light ______________ the specimen • Specimen must be _________. through thin

10. Light Microscope Image

11. Electron Microscope electrons dead • Use a beam of _______________- • The specimen must be _________ because an electron microscope is used in a vacuum with no _____ and no ________ air water

12. Scanning E.M. • Makes 3D pictures of surfaces • Magnify by _________ 60,000x

13. Scanning E.M. Image (3D)

14. Transmission E.M. 2D • Makes _____ pictures of thin slices • Magnify by __________ 100,000x

15. Transmission E.M. Image (2D) Butterfly Antenna

16. Other Microscopes • Probe Microscopes • Ion Microscopes • _____________ can be seen with these 2 types of microscopes. Atoms

17. Probe Microscope

18. Probe Microscope Image • Horse Hair

19. Ion Microscopes

20. Ion Microscope Images

21. Microscope Parts and Functions

22. 1. Eyepiece Magnifies by 10x

23. 2. Revolving Nose Piece Changes Objective Lens

24. 3. Low power Objective Lens Magnifies by 4x (Red)

25. 4. Medium Power Objective Lens Magnifies by 10x (yellow)

26. 5. High Power Objective Lens Magnifies by 40x (Blue)

27. 6. Stage Clips To hold slide in place

28. 7. Diaphragm Adjusts the amount of light

29. 8. Light Light to see specimen

30. 9. On/Off Switch Turns light on and off

31. 10. Arm To hold while carrying

32. 11. Stage To set slide on

33. 12. Course Adjustment To focus only when in Low Power

34. 13. Fine Adjustment To focus when using medium or high power

35. 14. Base • To hold while carrying

36. Both Arm A. Carry a microscope with ___________ hands – one hand holding the _______ And the other hand under the ___________ Always hold the microscope upright so the _____________ cannot fall out When you place a microscope at your table, make sure it is _______ from the edge If there is anything wrong with the microscope, or it is damaged in any way, let the _______________ know immediately. B. To clean the objective lenses and eyepiece, use _______________________ Base Eyepiece Away Teacher Lens Tissue

37. Prepare your slide • Plug the microscope in. Turn on the ______________ • Be sure the ___________ Power Objective Lens (_______) is clicked into place. • NOTE: Be sure to look at the microscope from the side when changing objective • lenses to avoid damaging the ________________ and _____________ • 4. Use coarse focus to raise the stage to its ________________ position • 5. Place the _______________ on the stage. • Look through the eyepiece and turn the __________________ (lowering the stage) • until the specimen is visible. • 7. Use the ____________ adjustment to bring your specimen into sharp focus. • You may wish to move the ________________ to adjust the amount of light. Light Low 4X Slide Lens Highest Slide Coarse Focus Fine Diaphragm

38. Focused 8. Make sure the specimen is ______________ 9. Switch to the ___________ Power Objective Lens ( ______) NOTE: Look at the microscope from the ____________ when you change lenses. 10. To avoid losing your focus and/or crushing the slide with the lens, never use the __________________ focus on medium or high power. Use the _____________ focus only to make the specimen visible. 11. Make sure the specimen is ______________. Look from the _________ as you change objective lenses and switch to the __________ Power Lens (________) 12. Use only the _____________ focus to make the specimen visible. 13. When finished, make sure the ___________ power objective lens is in place, wrap the __________ around the _________ of the microscope, cover, and place in the appropriate location Mid 10X Side Coarse Fine Focused Side High 40X Fine Low Cord Arm