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WP5 - MICROBIAL METABOLIC ACTIVITIES CNRS DR 12 - LMGEM

WP5 - MICROBIAL METABOLIC ACTIVITIES CNRS DR 12 - LMGEM. D. MARTY, J. GARCIN, C. TAMBURINI, A. BIANCHI. BIODEEP Meeting IX MILANO 17-19 May 2004. BACTERIAL ACTIVITIES. * STRICT ANAEROBIC ACTIVITIES * Methane Production (MP) * Sulfate Reduction (SR). * BACTERIAL PRODUCTION & ACTIVITY

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WP5 - MICROBIAL METABOLIC ACTIVITIES CNRS DR 12 - LMGEM

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  1. WP5 - MICROBIAL METABOLIC ACTIVITIESCNRS DR 12 - LMGEM D. MARTY, J. GARCIN, C. TAMBURINI, A. BIANCHI BIODEEP Meeting IX MILANO 17-19 May 2004 BACTERIAL ACTIVITIES • * STRICT ANAEROBIC ACTIVITIES • * Methane Production (MP) • * Sulfate Reduction (SR) * BACTERIAL PRODUCTION & ACTIVITY * Bacterial Production (BP) * Glutamate Uptake * Chemotrophic production * Hexadecane respiration

  2. Microbial activities measuredGlutamate uptake, Bacterial & Chemolithotrophic production, Hexadecane respiration *Heterotrophic bacterial production representative of the processes due to the whole heterotrophic microbial community *Chemolithotrophic production  biomass production by chemolitrophic organisms growing without organic enrichment, and using CO2 as C source for biomass production *Assimilation and respiration of glutamate  used as a rough index of the ratio linking bacterial biomass production processes to those used to produce energy *Hexadecane respiration  occasionally measured as a paragon of ability for microbial populations to mineralize hydrocarbon compounds

  3. Urania L'Atalante Discovery Bannock Bacterial production in the Upper interfaces relatively to the salinity pmol C l-1 h-1 0 10 20 30 40 50 0 5 10 15 20 S (%) 25 30 35 40

  4. Microbial activities within brinesHeterotrophic bacterial & Chemolithotrophic production 16 14 Heterotrophic bacterial production 12 Chemolitotrophic production 10 pmol C l-1 h-1 8 6 4 2 ND ND 0 Urania L’Atalante Discovery Bannock

  5. Microbial activities within brinesGlutamate assimilation and respiration, Hexadecane respiration Glutamate respiration Glutamate assimilation Hexadecane respiration 2 2 1,8 1,8 1,6 1,6 1,4 1,4 1,2 1,2 1 1 pmol C l-1 h-1 0,8 0,8 0,6 0,6 0,4 0,4 0,2 0,2 ND ND 0 0 Urania L’Atalante Discovery Bannock Urania L’Atalante Discovery Bannock

  6. Microbial activities in near-bottom waterHeterotrophic bacterial & Chemolithotrophic production 35 30 Heterotrophic bacterial production 25 Chemolitotrophic production 20 pmol C l-1 h-1 15 10 5 ND No NBB available ND 0 Urania L’Atalante Discovery Bannock

  7. Microbial activities in near-bottom waterGlutamate assimilation and respiration, Hexadecane respiration Glutamate respiration Glutamate assimilation Hexadecane respiration 4 4 3,5 3,5 3 3 2,5 2,5 2 pmol C l-1 h-1 2 1,5 1,5 1 1 0,5 0,5 No NBB available ND ND No NBB available 0 0 Urania L’Atalante Discovery Bannock Urania L’Atalante Discovery Bannock

  8. Microbial activities in the superficial sedimentsGlutamate assimilation and respiration, Hexadecane respiration Glutamate respiration with 1 µM initial concentration Glutamate respiration with 5 µM initial concentration 6000 Hexadecane respiration with 5 µM initial concentration 5000 4000 pmol C l-1 h-1 3000 2000 1000 0 Urania L’Atalante Discovery Bannock

  9. Toward the research of piezophile hydrocarbonoclastic bacteria from the Urania Basin *HP 1 10 mL inoculum brine + 100 mL sterilized brine + 40 µL Hexadecane + 20 mg (NH4)3PO4 + 8mM Na2S (ANOXIC CONDITIONS) *HP 2  10 mL inoculum interface + 100 mL sterilized interface + 40 µL Hexadecane + 20 mg (NH4)3PO4 *HP 3  10 mL inoculum brine + 100 mL ONR7a + 40 µL Hexadecane + 20 mg (NH4)3PO4 *HP 4   10 mL inoculum interface + 100 mL ONR7a + 40 µL Hexadecane + 20 mg (NH4)3PO4

  10. Toward the research of piezophile hydrocarbonoclastic bacteria from the Urania Basin

  11. Toward the research of piezophile hydrocarbonoclastic bacteria from the Urania Basin Pressure = 35 MPa HP bottles Agitation table

  12. Toward the research of piezophile hydrocarbonoclastic bacteria from the Urania Basin After several months of incubation  RNA (16S cDNA) extraction of subsamples of main culture show that an active population is living and growing in high-pressure conditions in each condition tested. 100bp Marker HP bottles 1 3 4 2 PCR gel of 16s cDNA amplification Results from Giuseppe D’Auria Cloning and sequencing are in progress…

  13. What about our article in AEM? Submission in September 2002 Response in January 2003  Major revisions : improve the English language, physical and chemical parameters, numbers of bacteria Potential authors : Gasparonni, D’Auria for TC, chemist?, May 2004 It would be very important to include other authors to improve the paper and corroborate some hypotheses we did

  14. What about our article in AEM? Data from cruise I Focus: Evidence of microbial life in DHABs

  15. What about our article in AEM?

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