bacterial genome sequencing

1. Bacterial Genome Sequencing SMRT Sequencing has long reads (average >15,000 bp, some reads >100,000 bp) and the highest consensus accuracy. It is especially helpful for genome de novo assembly. As we all know,repetitive stretches of DNA are abundant and are one of the main technical challenges that hinder accurate sequencing and genome assembly efforts. In the case of bacteria, the rRNA gene operon is often the largest region of repetitive sequence and range in size between 5 and 7 kb. Microbial whole genome sequencing by illumina HiSeq platforms utilizes sequencing by synthesis technology, that is limited by its read length, currently ranging from 50 to 300 bp, and as it requires PCR amplification of multiple DNA templates before sequencing, there is potential for base-composition bias which may bias the G+C content of the sequences. The advantage of SMRT long reads, which can overcome the problem of abnormal GC and high duplication of bacterial genomes, often assembled into a single contig. Among all assemblies, the PacBio assembly recovered the highest number of core and virulence proteins, and housekeeping genes based on whole-genome multilocus sequence typing. It is easier to achieve complete assembly or fine map, and help you rapidly advance your research to explore the genetic structure and functions. Learn more about bacterial genome sequencing at: https://www.cd-genomics.com/Bacterial-Whole-Genome-de-novo- Sequencing.html