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Supplemental Figure 4

Supplemental Figure 4. a. Succinate Succinate+ADP Succinate+Oligo Succinate+FCCP. b. Succinate Succinate+ADP Succinate+Oligo Succinate+FCCP. p <0.05. p <0.05.

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Supplemental Figure 4

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  1. Supplemental Figure 4 a Succinate Succinate+ADPSuccinate+OligoSuccinate+FCCP b Succinate Succinate+ADPSuccinate+OligoSuccinate+FCCP p<0.05 p<0.05 Figure S4: Bioenergetics of mitochondria from liver (A) and skeletal muscle (B) using succinate as a substrate. Bioenergetic determinations were performed with the Seahorse XF24. Mitochondria (10 μg) were attached to the surface of TC plates and then treated sequentially with ADP (final concentration; 0.1 mM), oligomycin (Oligo; 2.5 μg/mL), FCCP (8 μM), and antimycin A (4 μM) to test state 3, state 4, maximal respiration, and respiration independent of the electron transport chain, respectively. 1 μM rotenone was included in the reaction buffer to prevent reverse electron flow from complex II to complex I. OCR values were corrected for respiration not associated with the electron transport chain (antimycin A)Student t-test, n=4, mean±SEM.

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