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Mechanisms of gene silencing by double-stranded RNA

Mechanisms of gene silencing by double-stranded RNA. GENE SILENCING . Transcriptional level. Post-Transcriptional level. Structure of miRNA & siRNA. Pre- miRNA. P. OH. OH. P. siRNA. miRNA after processing . 22 ~ nt bases 5prime P & 3p 2 nt overhang

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Mechanisms of gene silencing by double-stranded RNA

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  1. Mechanisms of gene silencing by double-stranded RNA

  2. GENE SILENCING Transcriptional level Post-Transcriptional level

  3. Structure of miRNA &siRNA Pre-miRNA P OH OH P siRNA miRNA after processing • 22 ~ nt bases • 5prime P & 3p 2 nt overhang • Complimentrysequencs for target mRNA • Double stranded after processing by Dicer • 20-25 nt bases • 5prime P &3p 2nt overhang

  4. miRNA RanGTPase Exp-5 NPC Dicer

  5. Other sources of dsRNA Drosha miRna precursor Pri-miRna NPC DCR3/D2R2 Exp-5 DCR1 ATP+DCR1 Viral supressors Armitage R2D2 Ago Ago2 RISC Ago Ago2 miRNP AAA 7mG Ago Translational repression mRNA cleavege

  6. References • Gunter Meister & Thomas Tuschl, Mechanisms of gene silencing by double-stranded RNA, nature reviews, 2004, vol;431 343-349 • AlwinKöhle &Ed Hurt, Exporting RNA from the nucleus to the cytoplasm,nature reviews,2007, 1-13 • James C. et al Use of double-stranded RNA interference inDrosophila cell lines to dissect signal transduction pathways, PNAS, 2000, vol:97, 6499-6503

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