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The Chemistry of Photosynthetic Water Oxidation: From Fundamentals to Biofuels

Dive into the intricate world of photosynthetic water oxidation, from understanding the fundamentals to exploring its potential in biofuels production. Join the Kolling Laboratory in their research, focusing on the assembly of the oxygen-evolving complex, manganese concentration in cellular compartments, and metabolomic studies of Chlorella vulgaris. They seek dedicated students interested in challenging projects, grant writing, and long-term commitment. Explore extremophiles like Oscillatoria sp. and Thermosynechococcus elongatus to uncover temperature-dependent assembly processes. Investigate manganese distribution within Synechocystis sp. PCC 6803 and Chlamydomonas reinhardtii to unravel the photoassembly mechanism. Delve into the metabolomics of lipid synthesis pathways in Chlorella vulgaris, a potential biodiesel source. Experiment with O2 production, fluorescence, S-State trapping, and EPR spectroscopy methodologies to reveal the secrets of photosynthesis.

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The Chemistry of Photosynthetic Water Oxidation: From Fundamentals to Biofuels

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  1. Current Members Yasmine Zeid Ben Blodgett Cameron Buchman Courtney Nichols James Board Rebecca Mead Shaheed Elhamdani Evan Riley Matthew Thompson Former Members Chase Turner The Chemistry of Photosynthetic Water Oxidation: From Fundamentals to Biofuels Kolling Laboratory

  2. The lab has three main projects: • Assembly of the oxygen-evolving complex • [Mn2+] in compartments of phototrophs • Metabolomic studies of Chlorella vulgaris • We are looking for students who: • Would like to work >3 semesters • Are interested in writing grants • Enjoy challenging projects

  3. Project 1: OEC Photoassembly We are studying photoassembly in photosynthetic extremophiles that grow at very different temperatures. Oscillatoria sp. Thermosynechococcus elongatus cc cc www-dsv.cea.fr/var • Thermophile, 55°C • PSII crystal structure • Genome sequenced www-biol.paisley.ac.uk • Psychrophile, 12°C • Antarctic benthic mat

  4. Project 2: Compartmental [Mn2+] We are working to determine the concentration of Mn2+ in the different compartments of phototrophic prokaryotes and eukaryotes. This will help us to determine the mechanism of photoassembly. Synechocystis sp. PCC 6803 Chlamydomonas reinhardtii [Mn2+]? Lumen Lumen Cytoplasm Stroma Cytoplasm Chloroplast

  5. Project 3: Metabolomics We are developing a strategy to determine the partial metabolome of the lipid generation pathways in Chlorella vulgaris. This organism and its metabolism are of interest, because its lipids can be used to make biodiesel.

  6. Ferreira et al. (2004) Science A trip from a leaf to the oxygen-evolving complex. Barber & Iwata (2004) Science The oxygen-evolving complex!

  7. How do we probe photosynthesis? • O2 production • Fluorescence • S-State Trapping • EPR Spectroscopy

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