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Wu, Kuan-Yi

Wu, Kuan-Yi. Properties of minD/ ParA superfamily. Structurally similar ATPase activity Polymerization Other playes dynamic oscillation. (ParA). YFP-MinD/ MinE-CFP. The relationship between players. Placement of division sites C: inhibitor of septation D: membrane recruitment of C

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Wu, Kuan-Yi

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  1. Wu, Kuan-Yi

  2. Properties of minD/ ParA superfamily • Structurally similar • ATPase activity • Polymerization • Other playes • dynamic oscillation (ParA)

  3. YFP-MinD/ MinE-CFP The relationship between players Placement of division sites • C: inhibitor of septation • D: membrane recruitment of C • E: topological specificity DNA partition • A: recruited by B • B: recognize parS • S: cis-element in arrays

  4. MinC+MinD C MinC DMinD E MinE Division inhibition MinC concentration Zone of division inhibition MinE Septal FtsZ ring MinCDE polar zone pole-to-pole oscillation Placement of Z-ring

  5. 1 ADP MinD MinD - - - + + + 5 P MinC MinE 2 MinE 3 ATP ATP ATP ATP MinC MinE 4 MinC ATP ATP MinD MinD MinD MinD MinD Molecular mechanism of the oscillating Min system C-terminus

  6. In vitro In vivo MinD MinE ATP Phospholipid vesicle ParA ParB ATP

  7. A22 Localization of parA1scoe in different E.coli strains A22 treatment ΔmreB

  8. MC1000/ pSOT1013 Live cell image YFP DIC merge DAPI Oscillate with minD?  minD binding site Move independently membrane targeting? Existence of another proteins In the presence or absence of parB

  9. Test on ori/ ter localization YFP DIC-YFP DIC CFP IL2/ pLAU53 YFP DIC-CFP DIC CFP IL29/ pLAU53

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