Use of Primary Cells in Cell Culture and Drug Discovery

1. Use of Primary Cells in Cell Culture and Drug Discovery Revolutionizing Pharmacological Research Kosheeka: Primary Cell for Research www.kosheeka.com

2. Introduction to Primary Cells • Primary Cells are cells directly isolated from tissue, maintaining their physiological state. • Provide accurate models for human biology compared to immortalized cell lines. • This accuracy is crucial for understanding disease mechanisms and testing potential treatments. Image Credit: https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/primary-cell www.kosheeka.com

3. Human Biology Relevant Drug Interactions • Primary cells mimic human cell behavior more precisely, providing accurate results. • Used in studying drug interactions, toxicity, and efficacy. • Using primary cells, researchers can obtain more reliable data, leading to better drug development outcomes. www.kosheeka.com

4. Primary Cell Immortalization • Primary cells have a limited life span • They are able to maintain the physiological activity for longer duration • Immortalizing the cells extends the lifespan of primary cells by introducing specific genes. • It combines physiological relevance with the longevity of cell lines. • As a result, immortalized primary cells provide a stable and consistent model for long-term studies. Image Credit: https://acir.org/uploads/Immortalized_DCs.png www.kosheeka.com

5. Why are Primary cells demands increasing? • It is all because of FDA Modernization ACT 2.0 • The ACT is a legislative changes to modernize drug testing processes. • It has removed the manadate for including animal testing • he data obtained is more biologically relevant • This legislation supports the adoption of more accurate and innovative testing methods, which can expedite the approval of new drugs and therapies. www.kosheeka.com

6. Alternative drug testing systems using Primary Cells Researchers ar using three alternative drug testing systems: • 3D Bioprinting • Organ on chips • Organoids www.kosheeka.com

7. Primary cells in 3D bioprinting • 3D bioprinting allows researchers to create complex tissue structures • Results in more accurate tissue models • These models are essential for testing drug interactions • It closely resembles human organs • Enhances the reliability of preclinical testing and accelerates the development of effective treatments. www.kosheeka.com

8. Primary cells in Organ-on-Chip Technology • Organ-on Chip uses Microfluidic devices that mimic human organs using primary cells. • Provides a physiologically relevant environment for drug testing. • Allow researchers to observe how drugs interact with human tissues in real-time. • This technology holds great promise for improving the accuracy and efficiency of drug discovery processes. Image credits https://www.sciencedirect.com/science/article/pii/S0956566322006662 www.kosheeka.com

9. Primary cells help you Expedite your Drug discovery • Primary cells expedite research due to their relevance to human biology. • Provide more accurate data on drug interactions, toxicity, and efficacy • Faster, more reliable drug development cycles. • This efficiency can significantly reduce the time and cost associated with bringing new drugs to market. www.kosheeka.com

10. Conclusion • Primary cells play a crucial role in drug discovery by providing models that closely mimic human biology. • They enhance the accuracy of drug interactions studies • They can be easily incorporated in advanced technologies like 3D bioprinting and organ-on-chip to expedite research processes. By leveraging the unique advantages of primary cells, researchers can develop safer and more effective treatments, ultimately improving patient outcomes. www.kosheeka.com

11. TALK TO US • www.kosheeka.com • +91-9654321400 • info@kosheeka.com • A-102, Sector 5, Noida, 201301 Thank You www.kosheeka.com