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Bacteriology 4th Practical

PRESS F1 FOR GUIDEANCE. Bacteriology 4th Practical. MFSH 2003. Contents. Staphylococcus & Streptococcus Station 1: Staphylococcus VS. Streptococcus 3 Staphylococcus (aureus & epidermidis) 4 Station 2: Gram stain of Staphylococcus 5

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Bacteriology 4th Practical

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  1. PRESS F1 FOR GUIDEANCE Bacteriology 4th Practical MFSH 2003

  2. Contents Staphylococcus & Streptococcus Station 1: Staphylococcus VS. Streptococcus 3 Staphylococcus (aureus & epidermidis) 4 Station 2:Gram stain of Staphylococcus 5 Station 3: Gram stain of staphylococcus (pus) 6 Station 4: Staphylococcus growth on blood agar 7 Station 5: Deoxyribonulease test 8 Station 6: Mannitol sugar fermentation test 9 Station 7: Coagulase test 10 Station 8: Antibiotic sensitivity test, treatment & diseases 11 Station 9: Summary and revision 12 Streptococcus 13 Station 10: Gram stain of Streptococcus 14 Station 11: Gram stain of Streptococcus (pus) 15 Station 12: Gram stain of Streptococcus pneumoniae 16 Station 13: Gram Stain of Streptococcus pneumoniae 17 Station 14: Capsule stain of Streptococcus pneumoniae 18 Station 15: Alpha heamolytic Streptococci 19 Station 16: Beta heamolytic Streptococci 20 Station 17: Gama or non-heamolytic Streptocooci 21 Station 18: Optochin test 22 Station 19: Bacitracin test 23 Station 20: Capsule of Streptococcus Pyogens 24 Station 21: Lancefield Grouping of beta heamolytic Streptococci 25 Station 22: Treatment & diseases 26 Station 23: Summary and revision 27

  3. Station 1 Staphylococcus Streptococcus H2O2H2O2 H2O + O2(bubbles)H2O2 Saline Saline Saphylococcus Catalase +ve Streptococcus Catalase -ve • Bring 2 slides 2) Add saline 3) Put some of the bacteria 4) Add H2O2 5) See the difference 6) Take a look on the movie “Staph Vs Strept” included in the cd

  4. Staphylococcus

  5. Station 2

  6. Station 3 Staphylococci Pus cells

  7. Station 4 المفروض يكون اللون أكثر بياضاً من الموضح. و لكن ”لم تأخذ وقتها“ المفروض يكون اللون مائل للصفار و لكن ”لم تأخذ وقتها“

  8. Station 5 E.g. Staphylococcus aureus E.g. Staphylococcus epidermidis

  9. Station 6 MSA = Mannitol sugar agar E.g. Staphylococcus epidermidis E.g. Staphylococcus aureus Color changed to yellow Color not changed

  10. Station 7 Liquid state Coagulated state E.g. Staphylococcus aureus E.g. Staphylococcus epidermidis

  11. Station 8 Staph.aureus Infections:- 1- Pyogenic infections and abscesses 2- Osteomylitis 3- Food poisoning “milk products”  vomiting 4- Toxic shock syndrome in young women 5- SSSS “Staphylococcus Scalded Skin Syndrome” Treatment:- Drug of choice “Cloxacilin” if MRSA, (beta-lactamase producers) then we use “Vancomycin” • Staph.epidermidis & Staph.saprophyticus • Infections:- • Epidermidis  endocarditis with patients with catheters, cannulae & other artificial objects. • Saprophytics  Urinary tract infections in young female • Treatment:- • “Vancomycin” Not all the members of the same species share all properties together. For example, not all staphylococcus aureus are sensitive to the same antibiotics. (some of them are resistant to penicillin and others are not). Control When the distance between the control and the antibiotic is shorter than or equal to the distance between the antibiotic and the bacteria. A =< B The bacteria is called sensitive “to that anti-biotic” When the distance between the control and the antibiotic is longer than the distance between the antibiotic and the bacteria. A > B The bacteria is called resistant “to that anti-biotic” Sensitive Sensitive Resistant Sensitive Some “tribes” of Staphylococcus areus are sensitive to Penicillin (also Cloxacilin, Methicillin…) while others are not. Bacteria

  12. Station 9 Summary Test yourself Staph. aureus Staph. epidermidis +ve +ve Gram stain Color on Blood agar Mannitol fermentation test DNAse test Coagulase test Catalase Yellowish Whitish +ve “yellow” -ve “red” +ve -ve +ve -ve +ve +ve Best wishes

  13. Streptococcus

  14. Station 10

  15. Station 11

  16. Station 12

  17. Station 13

  18. Station 14 Organism (red) Capsule (transparent)

  19. Station 15 • Streptococcus can be divided upon their hemolytic activity into 3 groups: • α Hemolytic (e.g. Streptococcus pneumoniae) • β Hemolytic (e.g. Streptococcus pyogenes) • “These are also grouped by the LACEFIELD grouping {from A-H, K-W} as you will see later” • 3)γ or non Hemolytic (e.g. Streptococcus mutans)

  20. Station 16

  21. Station 17

  22. Station 18 There are colonies around the OPTOCHIN No colonies around the OPTOCHIN

  23. Station 19 There will be some colonies around the BACITRACIN strip. E.g. Streptococcus Pyogens Upon the Lancefield grouping

  24. Station 20

  25. Station 21

  26. Station 22 Alpha hemolytic streptococci (pnemococcus) Infection:- acute pnemonia sinusitis otitis media meningitis Treatment:- “Penecillin” Vaccination: By the Polysaccharide capsule Beta hemolytic streptococci gr.A (pyogens) Infection:- pharyngitis (sore throat) tonsilitis Treatment:- “Penecillin” • Some notes: • All Staph. :- VancomycinBut in non MRSA:- Cloxacilin is better • All Strept :- Penecilin

  27. Station 23 Summary Test yourself Strip. Pneumoniae Strip. Mutans Strip. Pyogens +ve +ve +ve Gram stain Color on Blood agar Color on Chocolate Hemolytic activity Lancefiel Grouping Optochin test Bacitracin test Catalase Green Clear No Chg. Green No Chg. No Chg. Alpha Beta Non NO Gp.A NO +ve -ve -ve -ve +ve -ve -ve -ve -ve Best wishes

  28. استغفر الله و أتوب إليه دعواتكم يا شباب

  29. I hope it was useful. A word can change the outcome of many things. So, if you either liked or didn’t like this project, why keep your thoughts to yourself ? Send us your comments and ideas to COORDINATOR1422@YAHOO.COM, It will mean too much to us, “even if it was just one word” THANX M. F. Shaheen 2003

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