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Bacteriology 5th Practical

PRESS F1 FOR GUIDEANCE. Bacteriology 5th Practical. MFSH 2003. Contents. Haemophilus, Mycobactria , Corynbacteriaua & Bordetella Haemophilus 3 Station 1: Gram stain of Haemophilus 4 Station 2: Gram stain of Haemophilus (pus) 5 Station 3: X,V factors & growth 6

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Bacteriology 5th Practical

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  1. PRESS F1 FOR GUIDEANCE Bacteriology 5th Practical MFSH 2003

  2. Contents Haemophilus, Mycobactria , Corynbacteriaua & Bordetella Haemophilus 3 Station 1:Gram stain of Haemophilus 4 Station 2: Gram stain of Haemophilus (pus) 5 Station 3: X,V factors & growth 6 Station 4: Growth of H.Influenzae on agar 7 Station 5: Satellitism 8 Station 6: Treatment & diseases 9 Station 7: Summary 10 Mycobacteria 11 Station 8: Ziehl-Neelsen stain 12 Station 9: Lowenstien Jensen media 13 Station 10: Treatment & diseases 14 Station 11: Summary 15 Cornybacteria 16 Station 12: Gram stain of Cornybacteria 17 Station 13: Albert stain of Cornybacteria 18 Station 14: Media used 19 Station 15: ELEK’s plate 20 Station 16: Treatment & diseases 21 Station 17: Summary 22 Bordetella 23 Station 18: Laboratory diagnosis 24 Station 19: Tratment & diseases 25

  3. Haemophilus

  4. Station 1 It can look like Bacillus or may look like coccus Coccobacilli

  5. Station 2

  6. Station 3 Different species of “Haemophilus” have different requirements to grow and make colonies. Some of them require X & V factors ( Haemophilus influenzae) Some of them need X factor only (Haemophilus ducreyi) Some of them need V factor only (Haemophilus parainfluenzae) X factor = heamatin V factor = Nicotinamide, Adenine & Dineiceotide This Bacteria grow when there are V & X factors. E.g. (H. Influenzae) This Bacteria grow when there is V factor. E.g. (H. Parainfluenzae)

  7. Station 4 Only X factor X+V factors No growth Growth No growth

  8. Station 5 X Factor present This is called Satellitism Staphylococcus strip Producing V Factor H.Influenzae growing here because of presence of both X & V factors

  9. Station 6 Haemophilus influenzae Infection:- meningitis acute epiglotitis in children (sore throught) osteomylitis & arthritis Cause bacteremia Treatment:- “Ampicilin” if beta lactamase producers  “Chloramphenicol” Vaccination:- Present by Polysaccharide capsule “serotype b” Haemophilus influenzae Infection:- respiratory tract infections No bacteremia

  10. Station 7 Summary Test yourself H. influenzae H. Parainfluenzae Gram stain Type Needs (X,V factors) Grows on Chocolate agar Grows on Blood agar -ve -ve Coccobacilli Coccobacilli X + V V only Yes Yes No No Best wishes

  11. Mycobacteria

  12. Station 8

  13. Station 9

  14. Station 10 Mycobacterium tuberculosis Infection:- 1-TB “lung infection with bloody cough” 2- Meningitis Treatment:- “Pyrazinamide + Isoniazid + Rifampicin”  2 months then, “Isoniazid + Rifampicin”  4 months Vaccination:- B.C.G “Bacille-Calmatte Guerin “10 years protection”

  15. Station 11 Summary Type “of Mycobacterium”: Stain used: Medium used: Acid-Fast Bacilli Ziehl-Neelsen Stain Lowenstein-Jensen medium Best wishes

  16. Corynebacteria

  17. Station 12

  18. Station 13

  19. Station 14 Black color Something like this There is growth but this is a wrong plate

  20. Station 15

  21. Station 16 Corynbacterium diphtheriae Infection:- Diphtheria “Bull neck appearance because of pharyngitis” affect: 1- nervous tissue damage (exotoxin) 2- heart muscle damage (exotoxin) 3- laryngeal obstruction 4- affect skin No bacteremia Treatment:- “Penecilin” Vaccination:- Present by toxoid “DPT vaccine” *D= diptheria toxoid *P= pertussis *T= tetanus

  22. Station 17 Summary Type & “gram” stain: Stains used: Media used: What is used for toxogenity test? Gram +ve bacilli Gram & Albert Potassium tellurite & Loeffler’s ELEK’s plate Best wishes

  23. Bordetella

  24. Station 18 (coocobacili)

  25. Station 19 • Bordetella pertussis • Infection:- • Whooping cough “due to an endotoxin” usually 1 year of age • complications: bronchopneumonia, bronchiatasis & subconjuctival hemorrhage • Treatment:- • “Erythromycin” but only affective at early stages • if beta lactamase producers  “Chloramphenicol” • No bacteremia • Vaccination:- • Present by whole killed bacteria “DPT”

  26. اللهم إنا نسألك زيادة في الدين وبركة في العمر وصحة في الجسد وسعة في الرزق وتوبة قبل الموت وشهادة عند الموت ومغفرة بعد الموت وعفوا عند الحساب وأمانا من العذاب ونصيبا من الجنة وارزقنا النظر إلى وجهك الكريم دعواتكم يا شباب

  27. I hope it was useful. A word can change the outcome of many things. So, if you either liked or didn’t like this project, why keep your thoughts to yourself ? Send us your comments and ideas to COORDINATOR1422@YAHOO.COM, It will mean too much to us, “even if it was just one word” THANX M. F. Shaheen 2003

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