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The Harderian Gland Revealed: Regeneration and Recovery of Chemosensory Function

The Harderian Gland Revealed: Regeneration and Recovery of Chemosensory Function. Andy Fraser Dr. Robert Mason & Chris Friesen Department of Zoology Oregon State University Corvallis, OR. Salamanders, for example, are able to regenerate limbs, tail, eyes, jaws, and heart.

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The Harderian Gland Revealed: Regeneration and Recovery of Chemosensory Function

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  1. The Harderian Gland Revealed: Regeneration and Recovery of Chemosensory Function Andy Fraser Dr. Robert Mason & Chris Friesen Department of Zoology Oregon State University Corvallis, OR

  2. Salamanders, for example, are able to regenerate limbs, tail, eyes, jaws, and heart. • We are largely unable to regenerate crucial organs and tissues. • Cures for debilitating diseases and injuries. • We know surprisingly little about how regeneration works. • It is crucial that we study new vertebrate models.

  3. Mating (~ 4 weeks) Summer feeding (~3 months) Winter Dormancy (~ 8 months)

  4. Detection of chemical cues Olfaction Volatile Vomerolfaction Nonvolatile Harderian Gland (HG) Solubizing agents Binding proteins Chemoreception

  5. Hypothesis: • The Harderian gland in the red sided garter snake is able to regenerate and regain its function over time.

  6. LD PO IO

  7. Experimental Design x Y Unilateral HDX N=28 x Y Bilateral HDX N=28 x Y Sham N=28

  8. 4 wks Unilateral HDX x 7 N = 21 x Bilateral HDX x 7 x x x 8 wks Sham 7 7 N = 21 7 x x x 12 wks 7 7 N = 21 x 7 x x 7 16 wks 7 x N = 21 7 x x 7 Experimental Design

  9. Behavior Trials: Y-Maze • Used to observe deficits in trailing behavior caused by removal of HG. • In this case we know that garter snakes will trail worms. Used coin flip to randomize which arm got trail.

  10. N=7 N=7 N=7 N=7 N=7 N=7 N=7 N=7 N=7 N=7 N=7 N=5

  11. Interpretation of Results • Two Hypotheses • 1. The HG regenerated more quickly than expected • 2. The IO portion of the HG hypertrophied or is sufficient.

  12. Acknowledgments • Dr. Robert Mason • Chris Friesen and Rocky Parker • Dr. Kevin Ahern • Howard Hughes Medical Institute

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