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Growth cones detect extracellular signals and guide axons to their targets

Growth cones detect extracellular signals and guide axons to their targets. Membrane signals and topography. Elf-1 (ephrinA2) repels anterior (nasal) axons. Flanagan and O’Leary Cell (1996). Ephrins and topographic patterning. Axon guidance at the midline. Trajectory of commisural axons.

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Growth cones detect extracellular signals and guide axons to their targets

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  1. Growth cones detect extracellular signals and guide axons to their targets

  2. Membrane signals and topography

  3. Elf-1 (ephrinA2) repels anterior (nasal) axons Flanagan and O’Leary Cell (1996)

  4. Ephrins and topographic patterning

  5. Axon guidance at the midline

  6. Trajectory of commisural axons

  7. A floorplate chemoattractant guides commisural axons (Tessier-Lavigne and Jessell)

  8. Netrin expression in the spinal cord (Serafini and Tessier-Lavigne)

  9. Netrin expressed in COS cells attracts commisural axons (Serafini and Tessier-Lavigne)

  10. Ventral spinal cord inhibits NGF-dependent sensory axons (Kolodkin and Goodman)

  11. Sema 3A expressed in COS cells inhibits NGF-dependent sensory axons (Kolodkin and Goodman)

  12. The slice overlay assay: a new technique to study apical dendrite patterning. Transgenic mice expressing GFP under b-actin promoter Oriented apical dendrite outgrowth Early postnatal cortical slices E15 embryos Non-oriented apical dendrite outgrowth Polleux and Ghosh

  13. Sema3A is expressed at high levels in the cortical plate during neuronal differentiation MZ CP 6a SP IZ SVZ VZ Modified from Giger et al. J. Comp. Neurol., 375, 378-92 (1996).

  14. Guidance of cortical axons by extracellular signals (Polleux and Ghosh)

  15. Sema3A repels cortical axons

  16. Pyramidal neuron morphology in Sema3A null mice

  17. Sema3A is an attractant for cortical dendrites

  18. Soluble Guanylate Cyclase co-localizes with MAP2 in the emerging apical dendrite

  19. Inhibition of soluble Guanylate Cyclase and PKG disrupts apical dendrite orientation DMSO ODQ SQ 22536 Rp-cAMPS Rp-8-pCPT-cGMPS P3 slice E15 GFP cells 4 div 150 microns

  20. Guanylate Cyclase Confers Distinct Responses to Axons and Dendrites Sema 3A Guanylate Cyclase

  21. A-P guidance in the spinal cord

  22. A-P guidance requires a diffusible signal (Zou and Tessier-Lavigne)

  23. Role of Wnts in A-P guidance

  24. Frizzled-3 is required for A-P guidance

  25. Topographic projections from retina to tectum

  26. Ephrins are expressed in a posterior-anterior gradient in the tectum (Flanagan, Bohnoeffer)

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