Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR

Aug 25, 2014

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Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR.

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Supplementary Table I . Gene-specific primers used for quantitatative real-time PCR

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Supplementary Table I. Gene-specific primers used for quantitatative real-time PCR

Supplementary data Table 1. PCR primers used to amplify TP53 Exons from genomic DNA

Supplementary data Table 1. PCR primers used to amplify TP53 Exons from genomic DNA. Arya et al. Supplementary data Table 1. MTT/ OD 570nM. Time/Days. Arya et al., Supplementary data Figure 1. 5 μ M Nutlin-3. 0.5 μ M Nutlin-3. DMSO. Untreated. DNA content. UM-SCC-5 (het).

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Real Time PCR

Real Time PCR. Dr Abida Raza Senior Scientist Molecular Diagnostics & Research Laboratory Nuclear Medicines Oncology & Radiotherapy Institute. Outline. Conventional PCR Real Time PCR Chemistries of Real Time PCR How to develop the assays Use of Real Time PCR at NORI

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Supplementary Table S1. List of oligonucleotide primers used for amplification

Supplementary Table S1. List of oligonucleotide primers used for amplification of RSV RNA1 and RNA2 segments. Supplementary Table S2. GenBank accession numbers of RSV RNA 1 and RNA2 segments used in the study. Jonson et al. Supplementary Fig. S1. (A) RNA2 ORF1. (B) RNA2 ORF2. BA2-JB.

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Supplementary Material 2. PCR primers used in this study.

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TABLE 5. Sequence of Primers used.

TABLE 5. Sequence of Primers used. FIGURE 5:CO-CULTIVATION OF RICE PLANT WITH CYANOBACTERIA.

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REAL TIME PCR

REAL TIME PCR. USING SYBR GREEN Courtesy of Dr. Hunt University of South Carolina. OVERVIEW. tissue. extract RNA. copy into cDNA (reverse transciptase). do real-time PCR. analyze results. REAL TIME PCR. kinetic approach early stages while still linear. www.biorad.com.

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REAL-TIME PCR

REAL-TIME PCR. APPLICATION PRINCIPLE INSTRUMENTATION INSTRUCTOR: EDDIE WAMPANDE. Roche lightcycler 480. Application of LC. Instrumentation Plate holder LED lamp CCD camera Computer— process the data- EXO. Absolute quantification of Nucleic acid Melting curve (SNP analysis) Tm

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Table S1 List of primers used for PCR and qPCR analyses

Supplementary data. Table S1 List of primers used for PCR and qPCR analyses. Fig S1. No LBL HBL. 1 5 10 1 5 10 (min). Coleoptile region from the top. a (Fig. 5 c). Molecular marker ( k Da ). 0-3 3-6 6-9 (mm). 180.

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Supplementary Materials and Methods Real-time RT-PCR

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Supplementary Table 2: Nucleotide sequence of primers used for PCR amplification

Supplementary Table 2: Nucleotide sequence of primers used for PCR amplification. Gene Accession no. Sequence cDNA Annealing Product Source

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Table S1. Primers used for semiquantitative RT-PCR

Table S1. Primers used for semiquantitative RT-PCR. 4. 4. 10. 10. 3. 3. 10. 10. 93.4. 96.6. 2. 2. 10. 10. 1. 1. 10. 10. 0. 0. 10. 10. 0. 1. 2. 3. 4. 0. 1. 2. 3. 4. 10. 10. 10. 10. 10. 10. 10. 10. 10. 10.

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Real-Time PCR

David A. Palmer, Ph.D. Technical Support, Bio-Rad Laboratories Adjunct Professor, Contra Costa College. Real-Time PCR. Objectives. This presentation will cover the following topics: What is real-time PCR used for? How does real-time PCR work? What instruments are used?

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Real-Time PCR. David A. Palmer, Ph.D. Technical Support, Bio-Rad Laboratories Adjunct Professor, Contra Costa College. Objectives. Today we’ll talk about Real-Time PCR: What is real-time PCR used for? How does real-time PCR work? What chemicals and instruments are used to detect DNA?

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Supplementary table 1: Primers used for PCR amplification

Primer name. Sequence 5`-3`. Accession Number. ChiF. ACCAAGCTACTCGCAAGAGG. CD485880. ChiR. CGGAAGCGCAGTAAGATGA. GluF. CATTGATATGACCTTGATCG. CD486342. GluR. GTGAGATATCCCTTGGATTG. PR1F. TGCCCAAGACTCACAACAAG. CK988133.1 (Patil et al. 2005). PR1R. GGCCTTCTCATTAACCCACA. Hist3F.

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Table S1. Primers and Probes Used in RT-PCR and qMSP

Supplementary Information. Table S1. Primers and Probes Used in RT-PCR and qMSP. Probe. Gene. Forward Primer. Reverse Primer. RT-PCR. GAPDH. GATATCTGGGTCATCGCTTC. TGAGTTTCCGTGAGGCTGAG. EMX2. GATATCTGGGTCATCGCTTC. TGAGTTTCCGTGAGGCTGAG. GAPDH EMX2. qPCR. Hs99999905_m1 Hs00244574_m1.

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Supplementary Table 1 . List of primers used for real-time PCR analysis

Supplementary Table 1 . List of primers used for real-time PCR analysis. --------------------------------------------------------------------------------------------------------------------------------------- Gene Forward primer Reverse primer

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Supplementary Table 1 Sequences of quantative RT-PCR primers

Supplementary Table 1 Sequences of quantative RT-PCR primers. Supplementary Table 2 Body weight, plasma parameters and blood pressure of E-DNI  B mice. Supplementary Table 3 Body weight and plasma parameters of E-DNI  B mice. Supplementary Figure 1. Lung. Aorta. Macrophages.

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Table S2. Sequences of primers used for RT-PCR

Supplementary Materials. Table S1. Features of patients with acute myocardial infarction or unstable angina and control subjects. AMI: acute myocardial infarction. * ANOVA test after the values were logarithm-transformed, # Chi-square test. ACEI: angiotensin convert enzyme inhibitors.

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BIOCHEMISTRY

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REAL TIME-PCR

REAL TIME-PCR. Biomaterials. TaqMan. 4. Institute for Genomics and Bioinformatics, TU Graz / Austria Dr. Juliane Strauss. Fluorescence resonance energy transfer.

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Used ABI 7500dx Real-Time PCR

Certified Genetool offers Used ABI 7500dx Real-Time PCR online. The ABI 7500 Fast Dx Real-Time PCR Instrument with SDS Software is a real-time nucleic acid amplification and five-color fluorescence detection system available for in vitro diagnostic use. For more information, please contact us. https://cgenetool.com/product/292/

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