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Particle Size Sizing Technique 1: Coulter principle

Particle Size Sizing Technique 1: Coulter principle. Kausar Ahmad Kulliyyah of Pharmacy, IIUM http://staff.iiu.edu.my/akausar. Electrical Sensing Zone Method A.k.a. the Coulter principle. Basic method of counting and sizing based on the detection and measurement of

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Particle Size Sizing Technique 1: Coulter principle

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  1. Particle Size Sizing Technique 1:Coulter principle Kausar Ahmad Kulliyyah of Pharmacy, IIUM http://staff.iiu.edu.my/akausar Physical Pharmacy 2

  2. Electrical Sensing Zone MethodA.k.a. the Coulter principle • Basic method of counting and sizing based on the detection and measurement of changes in electrical resistance, produced by a particle or biological cell, suspended in a conductive liquid, traversing through a small aperture. Physical Pharmacy 2

  3. Wallace Coulter - Coulter orifice (1956) • (as early as 1948) - measured changes in electrical conductance as cells suspended in saline passed through a small orifice • Cells are relatively poor conductors • Blood is a suspension of cells in plasma which is a relatively good conductor • Previously it was known that the cellular fraction of blood could be estimated from the conductance of blood • As the ratio of cells to plasma increases the conductance of blood decreases Physical Pharmacy 2

  4. From: http://www.cyto.purdue.edu/flowcyt/educate/ee520/sld008.htm Physical Pharmacy 2

  5. Principle Physical Pharmacy 2

  6. Interpreting the results Physical Pharmacy 2

  7. Advantages of Technique Physical Pharmacy 2

  8. Converting Signals to Particle Diameter Physical Pharmacy 2

  9. Calibration of instrument Physical Pharmacy 2

  10. Relationship between electrical signal and volume of particle Voltage proportional to volume of particle: U = constant x V constant = r0if / 2R4 U=amplitude of voltage pulse V=particle volume r0=electrical resistivity i= aperture current f= particle ‘shape’ factor R=aperture radius must not be dirty Physical Pharmacy 2

  11. Sample Concentration Physical Pharmacy 2

  12. Sample Condition Physical Pharmacy 2

  13. Aperture Physical Pharmacy 2

  14. Results Generated Physical Pharmacy 2

  15. Application Physical Pharmacy 2

  16. References JZ Knapp, TA Barber & A Lieberman, Liquid and Surface-Borne Particle Measurement Handbook, Marcel Dekker, New York (1996). T Allen, Particle Size Measurement 4th. Ed., Chapman and Hall, London (1990). Beckman-Coulter Multisizer 3 operation manual (lab) Physical Pharmacy 2

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