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MICROBIOLOGY TASK

MICROBIOLOGY TASK . MICROBIA ON ESTUARY WATER. Arranged by: Nur Laely Mubarokah Lita Purnamasari Andhis Exsa Seftilian Anindita safitri Rizqi Nur Amalia. What is estuary? Estuary is a buffer zone where freshwater meets saltwater.

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MICROBIOLOGY TASK

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  1. MICROBIOLOGY TASK MICROBIA ON ESTUARY WATER Arranged by: Nur Laely Mubarokah Lita Purnamasari Andhis Exsa Seftilian Anindita safitri Rizqi Nur Amalia

  2. What is estuary? Estuary is a bufferzone where freshwater meets saltwater Urban and farm sewage flow into coastal areas and estuaries. These waste waters, which arenot always welldepurated, contain many bacteria, some of which are fecal and pathogenic for man. After being dischargedinto the sea, some of these sewage bacteria are diluted and carried seaward.

  3. estuarine system is extremely complicated and dynamic.

  4. Plummer et al.(1987) clearly indicate that three groups of bacteria occur in estuarine waters: (1) free-living bacteria (2) bacteria attached (3) bacteria able to settle

  5. The abundance and occurrence of a special group of bacteria, capable of solubilizing or mobilizing insoluble phosphates were studied in estuarine environment, especially in sediments (A. Mukherjee, 2011)

  6. The estuarine part of the River Ganges, is one of the most dynamic, complex and vulnerable bioclimatic zones in a typical geographical location in the coastal region of Bay of Bengal. Samples were collected from 15 stations along there • And all of the stations have many microbes which living there

  7. Serial dilution of the samples were made with sterile water of appropriate salinities and plated in sterile petridishes containing Pikovskaya’s Agar

  8. Present investigation reveals that phosphate solubilizing bacteria and phosphatase activity exists in estuarine.

  9. Vibrio cholerae, Salmonella and Escherichia coli (Enterobacteriaceae) also indicated on estuary, it based on Colwell eksperiment on 1983.

  10. the estuary have large populations of sulfide-type magnetic bacteria such as Aquaspirillum serpens, A. dispar and A. itersoni (Dr. Richard P. Blakemore, 1990)

  11. The techniqueformonitoring microbial diversity Phylogenetic analysis of 16S rRNA genes usingdenaturing gradient gel electrophoresis (DGGE)

  12. The goals are: (1)Understand the geographic pattern of sediment bacterialcommunity in different layers along estuary; (2) Identify the major bacterial phylogeneticgroups and their diversity based on culture independent; and (3) Assess the impacts of environmentalchanges such asnutrient variations on the microbialcommunity structure using multivariate analysis.

  13. Referrence A. Mukherjee et al.2011. Abundance and occurrence of phosphate solubilizing bacteria and phosphatase in sediment of hooghly estuary, north east coast of bay of Bengal, India. Journal of Coastal Development ISSN : 1410-5217 15 ( 1): 9 – 16 Blakemore, Dr. Richard P.________ Isolation and growth of wild-type and mutant Magnetic bacteria. Blakemore; N00014-90-J- 1053; (cont'd) M. Pommepuy et al. Enteric bacteria surviv al Factors. Wat. Sci. Tech. 25(12):93-103 Rich,Jeremy J.2008.Anaerobic Ammonium Oxidation (Anammox) in Chesapeake Bay Sediments. Microb Ecol 55:311–320 Sudhanandh et al.2012. Distribution of potentially pathogenic enteric bacteria in coastal seawaters along the Southern Kerala coast, India. J. Environ. Biol.33, 61-66 Sun, Fu-Lin.2012. Spatial and vertical distribution of bacteria in the Pearl River estuary sediment. African Journal of Biotechnology 11(9:2256-2266 Ward, Bess B.2007. Ammonia-oxidizing bacterial community composition in estuarine and oceanic environments assessed using a functional gene microarray. EnvironmentalMicrobiology9(10), 2522–2538

  14. Thank you

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