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Project Motivation and Objectives

Electrical Actuation of Thrombin-Aptamer Molecule Therin Young 1 , Professor Shrotriya, Xiao Ma 2 Department of Mechanical Engineering, Iowa Sate University. Project Motivation and Objectives. Results and Discussion.

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Project Motivation and Objectives

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  1. Electrical Actuation of Thrombin-Aptamer MoleculeTherin Young1, Professor Shrotriya, Xiao Ma2Department of Mechanical Engineering, Iowa Sate University Project Motivation and Objectives Results and Discussion • Perform controlled actuation of aptamer and protein by applying specified voltages in an electro-chemical cell. • Use microcontact printing to print thrombin aptamer and thrombin protein onto gold surface. • Make Polydimethylsiloxane (PDMS) stamp using SU-8 photoresist master • Print alkanethiol • Image printings with atomic force microscope (AFM) • Alkanethiol printed on gold surface (in air) • Aptamer printed on gold surface (in air) • Aptamer-thrombin on gold surface (in air) • Electro-chemical Cell Setup • -300mV was applied Ag/AgCl Reference Electrode Pt. counter Electrode Sample with protein/aptamer complexes Experimental Methods Potentiostat • Making the PDMS stamp • 10:1 Mixture of silicon elastomer and curing agent • Pour over SU-8 photoresist master • Cure at 65̊C for 2 hours. • Microcontact print alkanethiol onto gold surface • Disperse thrombin aptamer • Expose it to thrombin protein • Apply -300mV, +300mV, and OCV using electrochemical measurement system • AFM Imaging to detect protein/aptamer binding induced height changes Materials 3’ • Gamry Instruments Reference 600 Potentiostat • SU-8 Photoresit Master • 18 μm x μm squares • 5.5 μm spacing • Gold-sputterd silicon wafers • Thrombin Aptamer (CTTAACT GTA GTACTG) • Human Thrombin Protein • 1-Hexadecanethiol (40mL ethanol solution, 123mg of hexadecanethiol) • Sylgard 184 Silicon Elastomer Base and Curing Agent 5’ Conclusions G-quadraplex structure of Thrombin Aptamer • Observations • Height changes occurred in each of the printings • Extreme height increases due to dust/impurities • Gold-sputtered silicon wafers gave clearer data then gold-coated glass slides • Use gold-coated silicon wafers for microcontact printing. • Improve quality and cleaning for the gold surface • Improve our electrical actuation process. D3100 Atomic Force Microscope AFM image of alkanethiol printed on gold-coated glass slide

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