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The Effects of Neo-synephrine on the 6-Day Chicken Embryo

Investigating Neo-synephrine's impact on 6-day chicken embryo hearts: heart rate & arrhythmias observed. Results support the hypothesis; caution needed in administration.

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The Effects of Neo-synephrine on the 6-Day Chicken Embryo

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  1. The Effects of Neo-synephrine on the 6-Day Chicken Embryo Sonya Park, Veronica Kvarta, and Dr. Jacqueline McLaughlin

  2. Chicken Heart Development The chicken heart develops from a series of fusions and differentiations. Four regions of the heart: • Conotruncus (ct) • Ventricle (v) • Atrium (a) • Sinus venosus (sv) 33 hour chicken embryo heart 72 hour chicken embryo heart http://www.lv.psu.edu/jxm57/chicklab/outline.html

  3. Autonomic Nervous System (ANS) • Involuntary • Consists of two divisions: Sympathetic Parasympathetic www.driesen.com/autonomic_nervous_system.htm

  4. Neo-synephrine Typically used as a nasal decongestant Some effects of Neo-synephrine: • Arrhythmias • Low blood pressure • Tremors • Restlessness • Excitability • Variation of potassium levels *Neo-synephrine medical warning

  5. Phenylephrine HCl • Active ingredient of Neo-synephrine • Alpha adrenergic agonist in sympathetic nervous system

  6. Purpose To determine the effects of various Neo-synephrine solutions on a 6-day in vitro chicken embryo heart. • Heart Rate (bpm) • Visible Arrhythmias

  7. Hypothesis The in vitro chicken embryo heart will experience visible arrhythmias along with a decrease in heart rate (bpm). 6-day in vitro chicken embryo

  8. Methods • Stock Solutions: • 0.125%, 0.1%, 0.075%, 0.05%, and 0.025% solutions of Neo-synephrine were made in chick saline • “Windowed” Egg: • obtained control in vivo heart rate • “Extracted” Egg: • obtained control in vitro heart rate • Administered Stock Solutions: • obtained experimental heart rate and noted arrhythmias

  9. RESULTSIn Vivo Heart Rates

  10. RESULTSIn Vitro Heart Rates

  11. RESULTSHeart Rates - 0.025% Beats per Minute

  12. RESULTSHeart Rates - 0.05% Beats per Minute

  13. RESULTSHeart Rates - 0.075% * * * Beats per Minute + + + * Tachycardia visible + Bradycardia visible

  14. RESULTSHeart Rates – 0.10% * * Beats per Minute * * + + * Tachycardia visible + Bradycardia visible

  15. RESULTSHeart Rates – 0.125% When the 0.125% solution was administered to each chicken embryo cardiac arrest occurred. Heart Rate = 0 bpm

  16. RESULTSAverageHeart Rates Beats per Minute In vivo In vitro 0.025% 0.05% 0.075% 0.10% 0.125%

  17. Conclusion • Due to the fact that noticeable arrhythmias occurred in the embryos’ heart, and there was a visible decrease in the heart rate, our results support our hypothesis. • Due to observations, it is obvious that Neo-synephrine must be carefully administered because high percentage dosages will cause dangerous arrhythmias in chicken embryos. • Further experiments could include: • Test lower concentrations of neo-synephrine to see if results are still consistent. • Administer an antiarrhythmic medication to see if arrhythmias occur and heart rate still decreases.

  18. Siamese Twins!!

  19. Q & A Please ask questions during this time. Thank you for listening to our presentation!

  20. Reference “Neo-synephrine.” Medications.com. Skylabs. 4 Feb. 2004 <http://medications.com/go/drug/Neosynephrine>. Armstrong, Lora L., Goldman, Morton P. et. al. Drug Information Handbook 2003-2004. Lexi-Comp, Incorporated, March 2003. Cruz, Y.P. 1993. Laboratory exercises in developmental biology. Academic Press, San Diego, California, 241 pages. [ISBN 0-12-198390-0] [book]

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