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HEALTHY FOOD FOR EUROPE

HEALTHY FOOD FOR EUROPE. AMARANTH. Amaranthus cruentus. LIPIDS IN SEED OIL. Amaranth seed oil HPLC of Lipid Classes (LLSD detection). TAG. Squalene. NI. TAG = triacylglycerols O – oleic acid C18:1 L – linoleic acid C18:2 P – palmitic acid C16:0

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HEALTHY FOOD FOR EUROPE

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  1. HEALTHY FOOD FOR EUROPE AMARANTH Amaranthus cruentus LIPIDS IN SEED OIL

  2. Amaranth seed oil HPLC of Lipid Classes (LLSD detection) TAG Squalene NI TAG = triacylglycerols O – oleic acid C18:1 L – linoleic acid C18:2 P – palmitic acid C16:0 NI – non identified (probably FFA) OOP LOO OOO LLO LLL OPP AMARANTH Sterols Squalene Standard Sterols (isolated fraction)

  3. GC of Amaranth seed oil (direct „cold on column injection”) Triacyloglycerols Squalene AMARANTH see next slide for TAG identification NI FFA FFA – Free Fatty Acids NI – non identified

  4. Amaranth seed oil – triacyloglycerols by GC (direct „cold on column injection”) PLP PLO PLL AMARANTH O– oleic acid C18:1 L – linoleic acid C18:2 P – palmitic acid C16:0 S – stearic acid C18:0 POO OLL OOL POP POS SOO OOO LLL SOS

  5. Fatty Acid Composition (GC) of Amaranth seed oil (pressed) C 18:1 9 C 16:0 C 18:2 C 18:0 Fatty acids C14:0 – myristic C16:0 – palmitic C16:1 – oleopalmitic C17:0 – margaric C18:0 – stearic C18:1 – oleic C18:2 – linoleic (9,12 all cis) C20:0 – arachidic C18:3– linolenic (9,12,15 all cis) C20:1 – eikozenoic C22:0 – behenic C24:0 – lignoceric AMARANTH C 17:1 C18:1 11 C 17:0 C 20:0 C 18:3 3 C 16:1 C 22:0 C 16:2 C 20:1 C 14:0 C 24:0

  6. Hydrocarbons isolated from Amaranth seed oil (HR GC, „cold on colum injection”) AMARANTH Squalene see next slide for traces

  7. Hydrocarbons isolated from Amaranth seed oil (including traces) Squalene AMARANTH hydrocarbons (traces)

  8. Tocopherols in Amaranth seed oil (HPLC/UV at 295 nm) Amaranth seed oil extracted with Carbon Dioxide (SFE) Alpha-T trienol Beta-T Alpha-T AMARANTH Amaranth seed oil extracted with hexane Beta-T Alpha-T trienol Alpha-T Delta-T trienol Delta-T Tocopherol Standards Alpha-T Beta-T Gamma-T Delta-T

  9. Supercritical Fluid Extraction of Amaranth seeds at 500C Squalene Pressure 250 bar FFA Triacylglycerols AMARANTH FFA Squalene Pressure 275 bar TAG TAG Pressure 320 bar FFA Squalene FFA – Free Fatty Acids

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