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Chapter 16.3

Automated DNA Sequencing. Chapter 16.3. AP Biology Fall 2010. Automated DNA Sequencing. Currently, laboratories use automated DNA sequencing to determine the unknown sequence of bases in a DNA sample. Automated DNA Sequencing. The machine builds DNA molecules but uses eight kinds of bases

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Chapter 16.3

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  1. Automated DNA Sequencing Chapter 16.3 AP Biology Fall 2010

  2. Automated DNA Sequencing • Currently, laboratories use automated DNA sequencing to determine the unknown sequence of bases in a DNA sample

  3. Automated DNA Sequencing • The machine builds DNA molecules but uses eight kinds of bases • Four normal • Four that are modified to fluoresce in laser light

  4. Automated DNA Sequencing • When a modified base is incorporated, DNA synthesis is halted • This produces tagged fragments of different lengths

  5. Gel Electrophoresis • The automated DNA sequencer separates the sets of fragments by gel electrophoresis

  6. Gel Electrophoresis • The “tag” base at the end of each fragment in the set is identified by the laser beam • The computer program in the machine assembles the information from all the nucleotides in the sample to reveal the entire DNA sequence • http://www.youtube.com/watch?v=oYpllbI0qF8

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