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The Effect of Beta Carotene on Plants Infected with Agrobacterium t umefaciens

The Effect of Beta Carotene on Plants Infected with Agrobacterium t umefaciens. Christina Adams Grade 9. Problem. Will Beta Carotene have any role in preventing the effect of Agrobacterium tumefaciens on violets?

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The Effect of Beta Carotene on Plants Infected with Agrobacterium t umefaciens

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  1. The Effect of Beta Carotene on Plants Infected with Agrobacteriumtumefaciens Christina Adams Grade 9

  2. Problem • Will Beta Carotene have any role in preventing the effect of Agrobacterium tumefaciens on violets? • I picked this experiment because of my interest in Botany and how different bacteria and solutions can affect the plant’s growth.

  3. Research • Beta Carotene is a red-orange pigment found in various types of plants. Beta Carotene is converted in the body to Vitamin A. • Agrobacteriumtumefaciens is the bacteria that causes tumor formation in over 140 different dicot plants (plants with 2 or more embyrotic leaves.) • A. tumefacienscan live freely in soil or inside plants as a parasite; Causes disease by transferring its own DNA into plants cells. • Most A. tumefaciensinfections caused by wounds in the plants (result from grafting together different plant stocks.)

  4. Hypothesis • If Beta Carotene is used, then it should have no effect on preventing damage from the Agrobacteriumtumefaciens.

  5. Materials • 24 violets • Beta Carotene solution • Disinfectant • Bunsen Burner • Inoculating Needle • AgrobacteriumTumefaciens

  6. Procedure • 8 plants were placed in each pot • Pot A is inoculated with A. tumefaciensand watered with 100ml of beta carotene twice weekly. • Pot B is inoculated with A. tumefaciensand watered with 100ml of distilled water twice weekly. • Pot C is not inoculated, but watered with 100ml of distilled water twice weekly. • The height of the plants will be documented over a 30-day period.

  7. Independent and Dependent Variables • Independent variable: what the seeds are germinated in • Dependent variable: the height and growth rate of the plants • Control: Group C (water control group)

  8. Data

  9. Data

  10. Data

  11. Conclusion • If Beta Carotene is used then it will have no effect on A. tumefaciensinfections. • This was not supported. • The control group had the highest growth rate followed by the beta carotene group. • The bacterial group had the lowest growth rate -Growth stopped after two weeks and plants began to brown

  12. Conclusion (cont) • How to improve experiment: - use different types of plants, such as monocot plants, and different types of solution to test the effectiveness of prevention. - Test the effectiveness of the Beta Carotene on infected plants in higher doses. - Test Beta Carotene on humans with cancer.

  13. Works Cited • Bochinski, Julianne Blair. The Complete Handbook of Science Fair Projects. N.p.: n.p., n.d. Print. • Helling, Christopher H. “How Effective is Beta Carotene in Fighting Cancer in Plants?” California State Science Fair 2008 Project Summary. N.p., n.d. Web. 5 Oct. 2011. • Tzfira, Tzvi, and VitalyCitovsky. “Taking Biology Lessons from a Bug.” The Agrobacterium- Plant Cell Interaction. N.p., n.d. Web. 6 Oct. 2011. • University of Minnesota- Department of Pediatrics. “Agrobacterium Infections in Humans.” University of Minnesota. N.p., n.d. Web. 6 Oct. 2011. • Various school students. “Do Plants Get Cancer?” Student Sheet. N.p., n.d. Web. 6 Oct. 2011. • Thank you for listening!

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