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Cell-Cell Communication, Sensing and Chemotaxis

Cell-Cell Communication, Sensing and Chemotaxis. N Kuldell for 20.020 Spring 2009. Heidelburg iGEM 2008: ecolicense to kill. Part 1: Sensing. Background information. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi. Cuttlefish: master of camouflage.

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Cell-Cell Communication, Sensing and Chemotaxis

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  1. Cell-Cell Communication, Sensing and Chemotaxis N Kuldell for 20.020 Spring 2009

  2. Heidelburg iGEM 2008: ecolicense to kill Part 1: Sensing Background information

  3. Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Cuttlefish: master of camouflage During the day they lie buried in the bottom of the ocean; at night they swim and hunt for food.

  4. AI-2 AI-1 Cell-Cell Communication natural context: Cuttlefish and Vibrio harveyi Few cells: Autoinducers diffuse away More cells: Autoinducers initiate signaling, transcription Photo from B. Bassler, Fig from: Genes & Dev. 2001. 15: 1468-1480 doi: 10.1101/gad.899601

  5. Cell-Cell Communication iGEM context: “prey” pathogen, “predator” killer Prey Cell LuxS= enzyme that produces autoinducer 2 (AI2)…diffuses through the media Predator Cell Nature (2002) 415: 545 LuxP, LuxQ = receptor that binds (AI2)…induces chemotaxis….how?

  6. AI-2 AI-1 Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor histidine kinase “RR” response regulator

  7. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output P TCS “SHK” sensor kinase “RR” response regulator Plant Physiol. (1998) 117: 723

  8. Sensing and Signaling Cascades natural context: “TCS” 2 component signaling Stimulus Activates SHK Transfers to RR Output Tar Che flagella P CCW, default, tumble CW, signal, swim TCS “SHK” sensor kinase “RR” response regulator J Bacteriol. (2000)182: 6865

  9. Sensing and Signaling Cascades iGEM context: chimeric receptor

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