1 / 14

Rhizome Project Meeting(1) 5-4-2011

Rhizome Project Meeting(1) 5-4-2011. Min- Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA. Approach. gene A. gene B. mRNA. microarray. RNA- seq. Intensity of spot. Number of sequence. Expression level. Work Flow. Flow cell.

libra
Download Presentation

Rhizome Project Meeting(1) 5-4-2011

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Rhizome Project Meeting(1)5-4-2011 Min-Jeong Kim Gang Lab Institute of Biological Chemistry Washington State University Pullman, WA

  2. Approach gene A gene B mRNA microarray RNA-seq Intensity of spot Number of sequence Expression level

  3. Work Flow Flow cell

  4. Total RNA Purification Tissuelyser II Qiagen RNA mini kit www.qiagen.com

  5. mRNA Isolation Total RNA www.invitrogen.com

  6. cDNA Library

  7. Cluster Generation with cBot (1) Attach DNA to flow cell surface Prepare DNA sample

  8. Cluster Generation with cBot (2) Bridge amplification Fragments become double stranded

  9. Sequencing and Data Analysis Align data Sequencing by synthesis(SBS)

  10. Progress on Specific Species * Some are not available in the database

  11. Transcriptome Analysis of Rhizome Parts

  12. Multiplexed RNA-seq Multiplexed sequencing Adding the sequence index to a library Clustering Pooling 5 replicates in a tube

  13. Interaction/Relationship with NCGR Sequencing Contig Library Flow cell Blast Database

  14. Thank You

More Related