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Fouling mechanism

Utilisation of bacteria for anti-fouling biofilm able to avoid the settlement and growth of micro&macro organisms. Fouling mechanism. 1: adherence of bact. Colony (Van der Waals) 2: anchoring (pilis) 3: polysaccharide matrix 4: M.O. Attachment 5: More fouling. State of research.

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Fouling mechanism

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  1. Utilisation of bacteria for anti-fouling biofilm able to avoid the settlement and growth of micro&macro organisms

  2. Fouling mechanism • 1: adherence of bact. Colony (Van der Waals) • 2: anchoring (pilis) • 3: polysaccharide matrix • 4: M.O. Attachment • 5: More fouling

  3. State of research... • Study of inhibitory effect of various bact. strains on various M.O. • Characterization of attachment mechanism of fouling agents • Characterization of inhibiting extracellular products

  4. Advantages/drawbacks

  5. Feasibility • Genetically: ? • Sea: very complex medium • Need significant upstream research (surface materials, ) • Need experimental validation of designed bacteria with many different conditions → more time than actual engineering ? • Lots of economical interest in this

  6. Bacteria used • Alteromonas • Pseudoalteromonas : extensively studied for its anti-fouling activity => Inhibit the settlement and growth of a large number of organisms

  7. Anti-fouling substance produced • Protein or peptide • Thermostable • Hydrophilic • >3500 daltons in molecular size • Inhibition of settlement by 2 ways: • direct: induce lysis in cultures • Indirect: emission from the bacteria of chemical products which inhibit growth and settlement

  8. Two possibilities • Continuous secretion of a given molecule which will avoid the deposit of molluscs on the hull • Secretion in response to a given input (e.g. shear stress)

  9. Description of the bioparts • Input: when bacteria sense an external input, triggering of the production of a certain molecule to avoid the deposit of plants and mollusc, for example in response to pressure => possibility to use luxR for example for the modelisation = inducible promoter, normally off, which turn on in response to an external input

  10. Description of the bioparts 2. Output: the bacteria can produce a molecule in order to kill molluscs attached or to prevent the adhesion. => possibility to use something like GFP as an output in our modelisation

  11. Description of the bioparts • 3. Filter: need to avoid the noise coherent feedforward loop with AND-gate. Input  fast degrading primary messenger  stable secondary messenger  AND-gate

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