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BACTERIOPLANKTON DYNAMICS DURING A MESOCOSM-SIMULATED OIL SPILL

BACTERIOPLANKTON DYNAMICS DURING A MESOCOSM-SIMULATED OIL SPILL Eva Teira 1 , JM Gasol 2 , I Lekunberri 2 , XAG Morán 3 , E Fernández 1 , XA Álvarez-Salgado 4 , F.G. Figueiras 4 1 Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, 36310-Vigo

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BACTERIOPLANKTON DYNAMICS DURING A MESOCOSM-SIMULATED OIL SPILL

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  1. BACTERIOPLANKTON DYNAMICS DURING A MESOCOSM-SIMULATED OIL SPILL Eva Teira1, JM Gasol2, I Lekunberri2, XAG Morán3, E Fernández1, XA Álvarez-Salgado4, F.G. Figueiras4 1 Departamento de Ecoloxía e Bioloxía Animal, Universidade de Vigo, 36310-Vigo 2 Departament de Biología Marina i Oceanografia, Institut de Ciències del Mar-CMIMA, CSIC, Pg.Marítim de la Barceloneta 37-49, 08003, Barcelona 3 Instituto Español de Oceanografía-Xixón, Camín de l’Arbeyal s/n, 33212, Xixón 4 Instituto de Investigaciones Marinas-IIM, CSIC, Eduardo Cabello 6, 36208, Vigo

  2. IMPRESIÓN (VEM2003-20021) “Impacto del vertido de hidrocarburos del PREStige sobre la red trófica microbiana planctÓNica”. Work programme 4: Impact on biologic systems. Impact assessment on biodiversity: population and communities distribution and abundance. Ecosystem restoration monitoring. Pelagic system.

  3. Polycyclic Aromatic hydrocarbons (PAHs) LMW (2-3 days) HMW (9 days)

  4. Alcanivorax Cycloclasticus Oleiphilus Oleispira Marine bacteria degrading petroleum hydrocarbons “professionals” marine hydrocarbonoclastic bacteria g-Proteobacteria G+ bacteria Planococcus LMW PAHs: Naphthalene Phenanthrene Anthracene

  5. Mesocosm experiment-IMPRESIÓN-1: Ría de Vigo, March 2005 Water filtered through 200 mm Temperature 10 ºC Chlorophyll-a ≈5 mg/l

  6. empty Bouzas bay 4 C1 1 L1 2 C2 3 H2 5 H1 6 L2 1.5m platform Mesocosm experiment-IMPRESIÓN-1: Ría de Vigo, March 2005 The experiment lasted 9 days C-Control L-Low PAHs concentration (»6 mg/L) H-High PAHs concentration (»15 mg/L)

  7. Methods • -Bacterial abundance:Direct microscopy on polycarbonate filters (DAPI-staining) • -Bacterial production:Rates of protein synthesis with 3H-Leucine • -Bacterial community composition:Fluorescent In Situ Hybridization • a-Proteobacteria (Alfa) • g-Proteobacteria (Gamma) • Cytophaga-Flexibacter-Bacteroidetes (CTB) • Cycloclasticus spp. (Cypu)

  8. 10 mm Fluorescence In Situ Hybridization (FISH): fluorescence-labeled oligonucleotide probes targeting the small subunit rRNA of specific prokaryotic groups. target probe

  9. PAHs addition Development and decay of a diatom bloom Lauderia annulata

  10. Bacterial abundance and production Day 1-3: bacterial abundance decrease in all the mesocosms Day 3: bacterial production is higher in H than in C Day 4-8: bacterial abundance and production is lower in H than in C

  11. Alfa (20%) CTB (35%) 10 mm Gamma (10%) Cypu (<1%) Bacterial composition-Day 0 -No significant differences in bacterial composition between C and H mesocosms -CTB and a-proteobacteria dominate the bacterial community -Cycloclasticus spp is undetectable. Control

  12. Alfa (10%) CTB (20%) 10 mm Gamma (20%) Cypu (7%) Bacterial composition-Day 4 * -Total detection is low in both C and L mesocosms (<50%) -Cycloclasticus spp comprise 7% of the total bacterial community in H mesocosms * High

  13. Alfa (15%) Alfa (30%) CTB (50%) CTB (60%) 10 mm 10 mm Gamma (30%) Gamma (15%) Cypu (1%) Cypu (n.d.) Bacterial composition-Day 8 CTB and Alfa Dominate in C CTB and Gamma Dominate in H * * Control High

  14. PAHs addition PAHs concentration and Cycloclasticus abundance Day 1-4: PAHs concentration rapidly decrease, and Cycloclasticus abundance increase Day 4-8: PAHs concentration drop to the levels in C, and Cycloclasticus abundance decrease

  15. Summary -Most of the PAHs disappeared within 3 days after the fuel addition. -Cycloclasticus abundance rapidly increased after the PAHs addition, reaching its maximum 3 days after the oil addition. -Cycloclasticus growth appears to be related to LMW PAHs degradation. -The bacterial community composition significantly changed 8 days after the oil addition: the g-Proteobacteria subclass predominate over a-Proteobacteria subclass.

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