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Lab 2: Organelles, Proteins, and SDS-PAGE

Lab 2: Organelles, Proteins, and SDS-PAGE. Dr. Sarah VanVickle -Chavez BIO 2960 Laboratory Spring 2013. Overview . Today we will load a gel and compare your gel to the sample gel. Each person will load one lane on the gels in the center of each bench.

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Lab 2: Organelles, Proteins, and SDS-PAGE

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  1. Lab 2: Organelles, Proteins, and SDS-PAGE Dr. Sarah VanVickle-Chavez BIO 2960 Laboratory Spring 2013

  2. Overview • Today we will load a gel and compare your gel to the sample gel. Each person will load one lane on the gels in the center of each bench. • While gels are running, we will do the PyMOL computer lab as a group demo. You should have Lab Report 1a complete before we leave today. • You can use your books and notes, but there is a brief computer tutorial on the NSLC website to help you with Lab Report 1b. • http://www.nslc.wustl.edu/courses/Bio2960/labs.html • Next week, there are three reports due at the beginning of class • 1a = proteins (PyMOL demo in class) • 1b = cell organelles (use your book/notes for this one) • 2 = SDS-PAGE

  3. 1. Protein Electrophoresis • SDS-PAGE stands for sodium dodecyl sulfate polyacrylamide gel electrophoresis

  4. SDS vs Native Gel How do you “see” your proteins?

  5. PyMOL • Remember: Amino acids have different properties based on the R-group • GAVLIMPFW = nonpolar • STNQYC = uncharged, polar • KRHDE = charged, polar

  6. Alpha helix

  7. Beta Sheet

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