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1. Stability of PyHA

08-04-2009 Jae Y Lee. 1. Stability of PyHA. Stabilities tests are on-going. Rat cortical astrocyte for 3 days in DMEM/F12K (with 10% FBS, 1% P/S/A) Biotinylated HA binding proteins, followed by PE-streptavidin treatment Cell culture incubator (37’C, 5% CO 2 )

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1. Stability of PyHA

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  1. 08-04-2009 Jae Y Lee 1. Stability of PyHA • Stabilities tests are on-going. • Rat cortical astrocyte for 3 days in DMEM/F12K (with 10% FBS, 1% P/S/A) • Biotinylated HA binding proteins, followed by PE-streptavidin treatment • Cell culture incubator (37’C, 5% CO2) • Replaced the solution every other day.

  2. 08-04-2009 Jae Y Lee Stability of PyHA Air – 2 weeks PBS – 3 months Hyase – 2 weeks

  3. 08-04-2009 Jae Y Lee 2. Long-term in vitro astrocyte culture : functional stability in terms of adhesion and migration 10 days 30 days • Previously cultured for 30 days • Fed every other day • 50 µm scale bars • On-going for longer time points 60 days 90 days

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