TPR domains

1. WXXXF motifs TPR domains N′ C′ 1 2 Absent in Pex5Sp

2. Trends in Plant Sci.6; 145, 2001 Peroxisome biogensis

4. PTS1 PEX5 Hsp70? PTS1 PEX5 Hsp90? m ? Trx m ? Trx m ? Trx m ? PTS1 PEX5 PEX5 PTS1 PEX5 PTS1 PEX5 cytoplasm PEX14 ? 13 17 2 10 12 peroxisome Redox shuttle PTS1

6. WXXXF motifs TPR domains N′ C′ 1 2 Absent in Pex5Sp Pex5pL translocartes Pex7p-PTS2 protein complex into peroxisomes via its Pex14p. : J. Biol. Chem. 275; 21703, 2000

11. Stress induces peroxisome biogenesis genes: EMBO J, 275: 21703, 2000 Fig. 2. Expression profile of AtPEX1 and AtPEX5 in seedlings. RNA from seedlings at the days indicated was hybridized with probes for AtPEX1, AtPEX5 and At malate synthase. Fig. 1. (A) AtPEX1 promoter and N-terminus of coding region. Italics show the additional 5' sequence derived from the 215 bp partial cDNA. The two possible ATG start codons are underlined. The amino acid sequence deduced from the cDNA clone is shown below the nucleotide sequence for the first and part of the second exon. The DDWE motif where the fusion to luciferase was made is shown in bold. (B) The fusion junction between PEX1 and luciferase. Fig. 3. H2O2 induces plant and animal peroxisome biogenesis genes. RNA used in (A) (northern blot) and (B) (RT–PCR) was obtained from control and 1 mM H2O2-treated Arabidopsis leaves, whereas in (C) (RT–PCR) the RNA is from control and 1 mM H2O2-treated chinese hamster ovary cells.

12. Fig. 5. H2O2 induces PEX1–LUC systemically. (A) Opposite leaves (arrows) were incubated with either 1 mM H2O2 (bottom leaf) or 1 mM H2O2 plus catalase (upper leaf) for 15 min. (B) The bottom leaf was incubated with the H2O2-regenerating system G/GO. Light emission was measured at the indicated times. Fig. 4. H2O2 rapidly induces AtPEX1–LUC gene expression. (A) Control plants (left) were sprayed with 1 mM H2O2 and light emission measured after 30 min (right). Clofibrate treatment (B) and senescence (C) were used as positive controls for peroxisome proliferation.

13. Induction of Peroxisome Biogensis by Stress Fig. 6.PEX1–LUC is induced by pathogen attack and wounding. (A) Pseudomonas syringae pv. tomato was inoculated (I) into the indicated leaf and the effect on PEX1–LUC expression compared with a control leaf inoculated with buffer only. Light emission was measured at the indicated times. (B–D) Wounded plants. (B) Whole plant before (left) and 90 min after wounding (right). (C) Close up of a leaf of the plant shown in (B). (D) Close up of leaf before and after wounding; different plant.