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Basic Electron Microscopy

Basic Electron Microscopy. Arthur Rowe. The Knowledge Base at a Simple Level. Introduction . These 3 presentations cover the fundamental theory of electron microscopy In presentation #1 we cover: the bits of optics we need to know production of an electron beam

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Basic Electron Microscopy

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  1. Basic Electron Microscopy Arthur Rowe The Knowledge Base at a Simple Level

  2. Introduction • These 3 presentations cover the fundamental theory of electron microscopy • In presentation #1 we cover: • the bits of optics we need to know • production of an electron beam • why we need vacuum & how we get it • the foundations of electron optics

  3. Sites for Electron Microscopy These sites contain useful information concerning most of the range of techniques available *************************************************************** Microscopy & Imaging: Resources on the WWW (University of Arizona) http://www.pharm.Arizona.edu/centers/tox_center/swehsc/exp_path/m-i_onw3.html Everything in sight (site ?), including the “Electron Microscopy Yellow Pages” ! **************************************************************

  4. ***************************************************** Gina Sosinsky’s EM Outreach program: http://em-outreach.sdsc.edu/ Based at the Supercomputing Center at San Diego, this site includes a very full set of Web-based lecture notes. Good text, but sadly, Gina has had problems getting many microscopists to agree to copyright release for their work, so for the moment the illustrations are rather minimal. One slight mis-statement, it is said that shadowing usually gives much worse resolution than negative staining. Not true. *****************************************************

  5. ***************************************************** Analytical Imaging Facility at Albert Einstein College of Medicine: http://www.aecom.yu.edu/aif/welcome.htm Concentrates heavily - as you would expect - on the analytical side of microscopy. All types are considered, light and electron microscopy being integrated together. As you might expect from the Albert Einstein, this site is a fantastic resource *****************************************************

  6. ***************************************************** Helen Saibil’s home page: http://www.cryst.bbk.ac.uk/~ubcg16z/saibil.htm Lovely images and some movies of selected protein-based systems, including chaperonin (GroEL - GroES) and pore-forming toxin (pneumolysin) *****************************************************

  7. ***************************************************** UNSW’s Image Gallery: http://srv.emunit.unsw.edu.au/html/gallery/gallery.htm Slanted towards histology in the biology section, but good images *****************************************************

  8. **************************************************** University of Sussex - EM site http://www.biols.susx.ac.uk/Home/Julian_Thorpe/cover.htm some good teaching material at a simple level, as well as some pretty images. ****************************************************

  9. and you can of course go on almost for ever, if you enter ‘electron microscopy’ into a search engine (SCIRUS is good for science) so now, on to the basics . . . . . .

  10. imaging with a simple lens

  11. the meaning of focal length

  12. when we image a simple point

  13. definition of resolution

  14. the electron gun

  15. wavelength & voltage

  16. why high vacuum ?

  17. how does a diffusion pump work?

  18. the electromagnetic lens

  19. the electromagnetic lens

  20. the transmission electron microscope

  21. The TEM Column • Gun emits electrons • Electric field accelerates • Magnetic (and electric) field control path of electrons • Electron wavelength @ 200KeV  2x10-12 m • Resolution normally achievable @ 200KeV  2 x 10-10 m  2Å

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