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Cohort F

Cohort F. Writing up the Research Plan. Introduction Slide Timeline of Experimental Plan Measurements at each embryo stage. This will show you where your two experiments will fit in

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Cohort F

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  1. Cohort F Writing up the Research Plan

  2. Introduction Slide • Timeline of Experimental Plan • Measurements at each embryo stage

  3. This will show you where your two experiments will fit in • Remember in order to properly experiment each experiment should be done three times to evaluate the significance of your observations Wrap Up Sessions: Conclusions and Posters Experiment 1: Dose-Response Relationships Experiment 2: Observational Relationships Lab Meeting 2 Lab Meeting 1 Two meetings before Exp2 Two meetings before Conclusions Scientific Training Meeting 2

  4. Dose Resonse Experimental Design: 1 Yellow dotted- yolk sac measurement Red box- yolk sac visual acuity Pink line- angle of body showing curvature of spine

  5. Table Describing Each Measurement

  6. Exp 2 Measurements • Measuring end of life in zebrafish development • Measuring growth defects • Measuring Cardiovascular anomalies (functional)

  7. Growth of Zebrafish during Development • You will measure embryos, in a length measurement. At each stage you will report this number. • One the y-axis you will show a measurement and x-axis, experimental conditions

  8. End Zebrafish Development • You will measure the number of embryos you start with, and then each day make this measurement, until early development. At each stage you will report this number. • One the y-axis you will show percent survival and x-axis experimental conditions

  9. Cardiovascular Development Measurements • You will measure heart rate, every 0.5 mins to generate a velocity curve • The y-axis will look the a same as the graph provided and the x-axis will read time, min

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