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Digital Pathology, An Introduction

Digital Pathology, An Introduction. From glass slides to digital files and there after. process. Scanning of glass slides by a scanner--- convert information to a digital format Navigate the digital slide using a software, from any power, on any area of interest, just like a glass slide

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Digital Pathology, An Introduction

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  1. Digital Pathology, An Introduction From glass slides to digital files and there after

  2. process • Scanning of glass slides by a scanner--- convert information to a digital format • Navigate the digital slide using a software, from any power, on any area of interest, just like a glass slide • Data analysis

  3. Applications • Remote access of slides: • Frozen Sections (night calls and remote sites) • Telepathology for 2nd opinion • Secondslide.com • pathxchange.com • Multi-institution collaborative studies • Retrospective and prospective collections of cases for TMA, case series studies, etc

  4. Real time consultation Bioimagene

  5. Clinical applications • Archival & viewing of data: • Tumor Boards • Quality Assurance (2nd read on QA cases) • Publications (? digital slide access as a part of case reports and case series in the future?) • Scan in key diagnostic slides for outside review cases, to avoid having to borrow back slides for clinical or academic needs. • Access old cases for comparison for frozen sections and current cases • Teaching sets for rare cases.

  6. Extractregion

  7. Image Management

  8. Digital IHC From Aperio tutorial,

  9. Bioimagene companion algorithm • Breast: ER, PR, Her2neu, ki67, p53 quantitation • Prostate: PIN-4 and Ki-67 • Brain: Ki67 • Heme: quantitation of CD138 labeled plasma cells in bone marrow samples. • Carcinoid: BioImagene’s Ki-67 carcinoid algorithm is an aid to pathologists and researchers in the detection and quantitation of stained cells in carcinoid tumors derived from gastrointestinal tissues.

  10. Practical issues • Cost (machine, software, storage & personal) • Time for scan: 15x15 mm tissue • 20x < 1min; 40 x 6-7 min • Memory: 20X scan: 250 MB; 40X scan: 800 MB • 1TB can hold 2,070 slides (assuming 500 MB/slide), or 4000 slides for 20x (250 MB)

  11. vendors • Aperio • Bioimagene • Olympus (nanozoomer) • Omnyx (UPMC+GE) • 3DHistech • Leica

  12. Issues for evaluation • Scanned image quality • Image management software (interface with LIS system if intend for clinical use) • Image analysis software • Technical support • Stability of vendor in the market • Good fit between your need and vendor’s strong points

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