1 / 20

Red Cross: 1-800-HELP-NOW or redcross AmeriCares: americares

Red Cross: 1-800-HELP-NOW or www.redcross.org AmeriCares: www.americares.org Episcopal Relief & Development: 1-800-334-7626 or www.er-d.org United Methodist Committee on Relief: 1-800-554-8583 or gbgm-umc.org/umcor/emergency/hurricanes/2005

lucy-fulton
Download Presentation

Red Cross: 1-800-HELP-NOW or redcross AmeriCares: americares

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Red Cross: 1-800-HELP-NOW or www.redcross.org AmeriCares: www.americares.org Episcopal Relief & Development: 1-800-334-7626 or www.er-d.org United Methodist Committee on Relief: 1-800-554-8583 or gbgm-umc.org/umcor/emergency/hurricanes/2005 Salvation Army: 1-800-SAL-ARMY or www.salvationarmyusa.org Catholic Charities: 1-800-919-9338 or www.catholiccharitiesusa.org FEMA Charity tips: www.fema.gov/rrr/help2.shtm National Voluntary Organizations Active in Disaster: www.nvoad.org Louisiana Society for the Prevention of Cruelty to Animals: www.la-spca.org Operation Blessing: 1-800-436-6348 or www.ob.org America's Second Harvest: 1-800-344-8070 or www.secondharvest.org Adventist Community Services: 1-800-381-7171 or www.adventist.communityservices.org Christian Disaster Response: 1-941-956-5183 or 1-941-551-9554 or www.cdresponse.org/cdrhome.html Christian Reformed World Relief Committee: 1-800-848-5818 or www.crwrc.org Church World Service: 1-800-297-1516 or www.churchworldservice.org Convoy of Hope: 1-417-823-8998 or www.convoyofhope.org Lutheran Disaster Response: 1-800-638-3522 or www.elca.org/disaster Mennonite Disaster Service: 1-717-859-2210 or www.mds.mennonite.net Nazarene Disaster Response: 1-888-256-5886 or www.nazarenedisasterresponse.org Presbyterian Disaster Assistance: 1-800-872-3283 or www.pcusa.org/pda Southern Baptist Convention - Disaster Relief: 1-800-462-8657, ext. 6440 or www.namb.net

  2. Any questions regarding material from the last lecture?

  3. Bioinformatic approaches are widely used to “guess” function since the structure of functionally-similar proteins is shared. It is dependent on the ability to query what is now very large databases of both primary and tertiary protein structures. BLAST analysis demo

  4. The function of a protein is determined by its structure. Ligand binding is specific and tight because of complimentary structures at interaction faces. Lots of noncovalent weak bonds are involved. “Hand-in-glove.

  5. It is the binding site (pocket) that allows a protein to interact with a specific ligand. It does so with stereo-specificity

  6. Example: immunoglobulin, binds antigen tightly to inactivate or mark for destruction

  7. 3 ways that binding pockets favor reactions, but all three work by stabilizing a transition state. Learn terms in table 4-1!!! Don’t sweat the details fig 4-34, Lysozyme mechanism

  8. “Extensions” to proteins give them special functions. Take for example, retinal of rhodopsin and heme of hemoglobin. Vitamin A cis retinal (photoisomerization to all-trans retinal) http://www.elmhurst.edu/~chm/vchembook/533cistrans.html Some extensions bond with the substrate Biotin, metals so-called co-enzymes

  9. Take for example: hemoglobin, four oxygen carrier, heterotetramer Four O2; each affect binding of another

  10. How are proteins controlled? Catalytic properties controlled *Feedback inhibition Allostery & conformational changes Most enzymes are allosteric 2 conform. *but positive feedback also occurs such as ADP stimulation of glucose breakdown see Fig. 4-40

  11. Allostery, feedback inhibition- example, aspartyl transcarbamoylase and cytosine triphosphate, a product of this pathway. ATC makes the nucleotide ring for pyrimidine. CTP is a product of this pathway

  12. Phosphorylation can control protein activity by triggering a conformational change.

  13. GTP binding proteins are self regulating. They are controlled by the loss (hydolysis) and gain (GTP/GDP exchange) of a phosphate group. The additional phosphate (highly negative) can cause a major conformational change. This is an amplification mechanism, like a lever.

  14. Nucleotide binding and hydrolysis allows motor proteins to produce large movements. Same idea: phosphate alters gross conformation Essentially irreversible

  15. NOW, let’s put “working proteins” in their setting.

  16. Compartmentalization solved many problems. Surface area vs. volume Concentration dependence Toxic reaction products Selective barriers Stored energy across compartments Dynamic: Information gate, site of transport, flux for movement

  17. The PM has properties that make it an unusual barrier. Fluid mosaic, 50 atoms thick. Lipid bilayer

  18. Phospholipids are amphipathic.

More Related