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Effects of ALSV Inoculation on Soybean Leaves Over Time

This supplemental figure set illustrates leaf changes in soybean plants following ALSV inoculation at different nodes over 4 weeks, examining various gene expressions involved in plant defense mechanisms.

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Effects of ALSV Inoculation on Soybean Leaves Over Time

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  1. Supplemental Figure 1 True leaf from 2nd to 7th nodes (a) M 1st (IN) IN 2nd 3rd 4th 5th 6th 7th PC ALSV-RNA2 7 PsEF1-a 6 1 2 3 5 True leaf from 2nd to 7th nodes ALSV inoculation 4 2nd 3rd 4th 5th 6th 7th 3 Mock 2 Grown for 4 weeks 1 1 wtALSV 4 5 (b) (c) Wild-type ALSV ALSV:soyPDS 21 dpi (4 weeks after ALSV inoculation)

  2. Supplemental Figure 2 (a) (c) ALSV:LOX ALSV:AOC ALSV:AOS ALSV:OPR wtALSV Mock M NS S ALSV:LOX LOX ALSV-RNA2 EF1-α ALSV-CP NS S ALSV:AOS M (b) AOS Mock wtALSV ALSV:LOX EF1-α M NS S ALSV:AOC AOC EF1-α ALSV:AOS ALSV:AOC ALSV:OPR NS S ALSV:OPR M OPR EF1-α

  3. Supplemental Figure 3 LOX AOS AOC OPR EF1-α Suppressor MeJA Coronatine N 0 50 100 200 50 100 200 50 100 200

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