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“CRISPR genome editing” Precise gene regulation/modification using the simple CRISPR/Cas9 system. Jason Kerkvliet , M.S. Research Associate II Hoppe lab. March 4 , 2014. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system.
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“CRISPR genome editing”Precise gene regulation/modification using the simple CRISPR/Cas9 system Jason Kerkvliet, M.S. Research Associate II Hoppe lab March 4, 2014
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system
DNA repair mechanisms: gene editing efficiency is enhanced by creating breaks in DNA
How does one use CRISPR? For NHEJ • CRISPR vector $65 • 4 (2 sgRNA sequences) oligos $40 • BbsI$70 • Total = $175
CRISPR gRNA design tool crispr.mit.edu
CRISPR/Cas9 can be used to edit more than one gene in parallel Wang et al., Cell, 2013
Gene knock-down (siRNA) versus gene knock out Shalem et al., Science 2014
Eliciting gene function using a CRISPR mediated genome screen Shalem et al., Science 2014