180 likes | 412 Views
Na+. Na+. Na+. Positively charged counterions. Na+. Na+. Na+. Na+. Na+. Na+. Na+. Rinse in buffer, add ionic compounds. Positive charges (triangles) stick. Negatives (squares) and neutrals (pentagons) do not stick. Na+. Na+. Na+. Na+. Na+. Na+. Negatively charged groups (red).
E N D
Na+ Na+ Na+ Positively charged counterions Na+ Na+ Na+ Na+ Na+ Na+ Na+ Rinse in buffer, add ionic compounds. Positive charges (triangles) stick. Negatives (squares) and neutrals (pentagons) do not stick Na+ Na+ Na+ Na+ Na+ Na+ Negatively charged groups (red) Sodium ions replaced by positively charged ions Rinse Na+ Na+ Na+ Na+ Cation Exchange Chromatography
From Wikipedia Commons, Author=Jeffrey M. Vinocur http://en.wikipedia.org/wiki/File:Gel_electrophoresis_apparatus.JPG
From Wikipedia Commons. Author = Dr d12 http://en.wikipedia.org/wiki/File:DNAgel4wiki.png