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Field of View of a Microscope. Specimens are measured in microns (µm)! 1 millimeter = 1000 microns (micro meters). If this caterpillar is 20mm long, how many microns is it?. (20mm)(1000) = 20,000 microns. Which magnification will show more space, 40X or 100X?.
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Specimens are measured in microns (µm)! 1 millimeter = 1000 microns (micro meters) If this caterpillar is 20mm long, how many microns is it? (20mm)(1000) = 20,000 microns
Which magnification will show more space, 40X or 100X? 40X will show more than 100X
Ruler at 40X How many mm across? 3mm How many microns across? 3,000 µm 3 X 1000 = 3,000 µm across
Ruler at 100X • How many millimeters? Microns?
Use the equation on your lab sheet to calculate the field of view at 400X High power magnification Low power magnification Low power diameter High power diameter =
400 40 3 mm X (40)(3mm) = (400)(X) = 120mm = 400X 120mm 400 0.3mm =
At 400X you only see 0.3 mm across How many microns is that? 0.3mm X 1000 = ? 300 µm
Estimating the size of objects If the field of view is 600 microns across, how far across is one specimen?
How many specimen can fit across? If you can fit 6 across 600 microns, how far across is one circle? Diameter / # of objects 600 microns/6 circles = 100 microns each
Use the same method to estimate how wide a blood cell and a piece of hair are 400X
40X 100X 400X
40X 100X 400X