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Growth and Cell Division

Growth and Cell Division. Growth: Not size, but number of cells Bacteria: Binary fission Yeast: Budding. Cell Division. Bacterial Growth Curve. Lag phase Log phase Stationary phase Death phase. Measuring Bacterial Concentration. Serial dilution/ Standard plate counts

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Growth and Cell Division

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  1. Growth and Cell Division • Growth: • Not size, but number of cells • Bacteria: • Binary fission • Yeast: • Budding

  2. Cell Division

  3. Bacterial Growth Curve • Lag phase • Log phase • Stationary phase • Death phase

  4. Measuring Bacterial Concentration • Serial dilution/ Standard plate counts • CFU = Colony Forming Unit • CFU/ml

  5. Measuring Bacterial Growth • Direct microscopic counts

  6. Measuring Bacterial Growth • Most probable number

  7. Measuring Bacterial Growth • Filtration • Turbidity (Spectrophotometry)

  8. Physical Factors Affecting Bacterial Growth • pH • Temperature

  9. Physical Factors Affecting Bacterial Growth • Oxygen • Moisture

  10. Physical Factors Affecting Bacterial Growth • Osmotic pressure • Salt • Radiation

  11. Nutritional Factors Affecting Bacterial Growth • Carbon source • Nitrogen source • Sulfur/ phosphor • Trace elements • Vitamins • Nutritional complexity • Enzymes: • Extracellular • Periplasmic • Cytoplasmic

  12. Sporulation • Endospores • Other sporelike bacterial structures

  13. Culturing Bacteria — Streak Plates • Streak plate method

  14. Culturing Bacteria — Pour Plates • Pour plate method

  15. Culture Media Purposes • Selective media • Differential media • Enrichment media

  16. Culture Conditions — O2 • Controlling oxygen content

  17. Culture conditions • Maintaining cultures • Preserved cultures • Diagnostic tests • Limitations

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