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NRE pLexA-p65 LexA p65 MPSV pL6NLuc LexA Firefly Luc pRL-Tk Tk Renilla Luc + + + + pL6NLuc - + + + pLexA-p65 -- NRF(1-380) NRF(1-690) NRF expression vectors Figure 1

Figure 2 A p65 bound p50 bound B NRF(1-380)TAP + p65RHD NRF(1-380)TAP + p65 NRF(1-380)TAP + p65RHD NRF(1-380)TAP + p65 NRF(1-380)TAP p65 endogenous p65 p65 RHD bound total

spot peptide sequence 1 1MDELFPLIFPAEPAQ15 24LFPLIFPAEPAQASG18 3 7LIFPAEPAQASGPYV21 410PAEPAQASGPYVEII24 . . Figure 3 A B Control Spots 1 26 51 76 RRL 1 26 51 76 NRF-Strep 85 199 114 Spots 1 26 51 76 309 274 246

peptide length • spot peptide sequence • 1 88HELVGKDC95 • 289ELVGKDCR96 • 3 90LVGKDCRD97 • 87PHELVGKDC95 • 88HELVGKDCR96 • 89ELVGKDCRD97 • 86HPHELVGKDC95 • 87PHELVGKDCR96 • 88HELVGKDCRD97 8 aa 8 aa 8 aa .. 9 aa 9 aa 9 aa .. 10 aa 10 aa 10 aa .. 13 11 14 9 12 15 10 Figure 4 A 81 PPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQ 114 B NRF-Strep peptide length spots 8 8-9 aa 1 9-10 aa 26 10-11 aa 51 11-12 aa 76 12-13 aa 101 13-14 aa 126 14-15 aa 151 15 aa 176

spot peptide sequence 1 199VNRNSGSCLGGDEIF213 2200NRNSGSCLGGDEIFL214 3 201RNSGSCLGGDEIFLL215 . . Figure 5 A 199 VNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHR 246 B NRF-Strep Spots 1 26

spot peptide sequence 1 274RPSDRELSEPMEFQY288 2 274APSDRELSEPMEFQY288 3 274RASDRELSEPMEFQY288 4 274RPADRELSEPMEFQY288 18 277DRELSEPMEFQYLPD291 19 277ARELSEPMEFQYLPD291 20 277DAELSEPMEFQYLPD291 21 277DRALSEPMEFQYLPD291 . . . . NRF-Strep spots 1 26 51 76 101 Figure 6 A 274 RPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTY 306 B

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