1 / 14

Factor V Leiden Detection and Genotyping

Factor V Leiden Detection and Genotyping . August 6, 2008 Marylin Bicak. Objective . 1. To provide an overview of the Factor V Leiden assay 2. To explain the pathophysiology and epidemiology of the Factor V Leiden mutation. 3. To evaluate the efficacy of the assay. Pathophysiology.

madge
Download Presentation

Factor V Leiden Detection and Genotyping

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Factor V Leiden Detection and Genotyping August 6, 2008 Marylin Bicak

  2. Objective • 1. To provide an overview of the Factor V Leiden assay • 2. To explain the pathophysiology and epidemiology of the Factor V Leiden mutation. • 3. To evaluate the efficacy of the assay.

  3. Pathophysiology • inherited condition • autosomal dominant • chromosome 1, gene F5 • Single point mutation, G1691A (arginine to glutamine at 506th amino acid • mutation prevents inactivation of factor V in clotting process (resistant to inactivation by Protein C) • overproduction of thrombin= excess fibrin formation • excess clotting (DVT and PE)

  4. Epidemiology • Most common inherited coagulation disorder in U.S. • 5% Caucasians • 2% Hispanics • 1.2% African Americans • <0.5% Asian Americans • Heterozygous= 3-8 fold increase risk of thrombosis • Homozygous= 30-140 increase risk of thrombosis • risk factors • treatment

  5. Factor V Leiden Assay • 1. Extraction- MagNA Pure Compact Nucleic Acid Isolation Kit and instrument (Roche Diagnostics) • 2. Amplification/Detection/Genotyping- Factor V Leiden Kit and LightCycler 2.0 instrument (Roche Diagnostics)

  6. Extraction • Specimen- EDTA whole blood (200 ul whole blood= 100 ul purified product) • MagNA Pure Kit- pre-sealed reagent cartridge; disposable pipette tip tray assembly; sample tube; elution tube • MagNA Pure Compact Instrument-automated method based on magnetic glass particle technology

  7. Principle of Magnetic Glass Particle Technology • Four step process: 1-lysis; 2-bind to magnetic particles; 3-wash; 4-elution

  8. Nucleic Acid Isolation

  9. Amplification-real-time PCR • target- 222 bp fragment of Factor V gene • Factor V Leiden kit- master mix reagents (primers and probes) • LightCycler 2.0 instrument- rapid- 45 cycles (30 min) • Cycle temperatures- denaturation 95C; annealing 60C; elongation 72C • thermal cycler- heat/ambient air cycle • reaction vessel- 20 ul glass capillary

  10. Detection • FRET (fluorescence resonance energy transfer)

  11. Genotyping • Melting Curve Analysis

  12. Summary • Limitations- technical process/ physical space/ instrumentation • Erroneous results- a. false positive (3 rare mutations span same mutation probe) and b. patient sample with elevated WBC • Overall, innovative system and important diagnostic tool for clinical lab

More Related