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Cell differentiation basing on ACTB expression

Cell differentiation basing on ACTB expression. AIM . Detect mouse and human ACTB gene in two co-cultured cell liens Cell Lines: MEF – Mus muscullus embroynic fibroblasts

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Cell differentiation basing on ACTB expression

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  1. Cell differentiationbasing on ACTB expression

  2. AIM • Detect mouse and human ACTB gene in two co-cultured cell liens Cell Lines: MEF – Musmuscullusembroynic fibroblasts Cells infected with SV40 polyomavirus. LargeT antigen(protein) is believed to bind and limit p53 activity – transcription factor responsible for cell death

  3. AIM • Detect mouse and human ACTB gene in two co-cultured cell liens Cell Lines: BjhTERT – Homo sapiens sapiens fibroblast immortalised with hTERT(human telomere reverse transcriptase)

  4. AGCCTCGCCTTTGCCTTCCTTTTACGACCTCAATGCTGCTGCTGTACTACTCTTCGCCCCGCGAGCACAG TTACACCCTTTCTTTGACAATCCGAGTAGTCTTTGTGCGTCTATTTAGTGGAGCCGCAACTATCTTCTTTGACTGTTACTGAGCTGCGTT Cy3 Cy5 CCTCAATGCTGCTGCTGTACTAC TGCGTCTATTTAGTGGAGCC

  5. No primer given for mouse ACTB padlock. We will use random priming ! (random decamers : 10 bases oligos)

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