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Punnett Squares. Step by step how to guide. Putting it together. Alleles represented by letters Capital letters = dominant (T) Lowercase letters = recessive (t) Two letters combined = trait TT, Tt, tt One from mom and one from dad Homozygous = both letters same TT or tt
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Punnett Squares Step by step how to guide
Putting it together • Alleles represented by letters • Capital letters = dominant (T) • Lowercase letters = recessive (t) • Two letters combined = trait • TT, Tt, tt • One from mom and one from dad • Homozygous = both letters same • TT or tt • Heterozygous = both letters differ • Tt
Punnett Squares • A diagram that shows all possible outcomes of a genetic cross • Can be used to predict probabilities • Phenotype – an observable trait • Tall or short • Genotype – genetic make-up or combination of alleles • TT Tt tt
Initial Steps (for a monohybrid cross) 1. Identify the Parents being used in the cross • Homozygous or heterozygous • TT, Tt, tt? • Most important step! 2. Segregate the alleles in each set of genes for each parent • Show meiosis creating the haploid cells
Parent: (P) Tt x Tt Heterozygous Tall crossed with Heterozygous Tall Segregation of alleles: T t T t x Draw the Square: T t T Place the parents: t
T t T T T T t T t t t t
Tt x Tt What’s next? Ratios T t Genotypic Ratio: Ratio of allele combinations T T T T t TT = Tt = tt = 1 2 1 1:2:1 t T t t t Phenotypic Ratio: 3 1 3:1 Tall = Short = Ratio of traits
Examples for you to work out! • Tt x tt -be sure to show both types of ratios • Cross a homozygous tall plant with a short plant -be sure to show both types of ratios
Initial Steps (for a dihybrid cross) 1. Identify the Parents being used in the cross and figure out the combo of both traits. • Homozygous or heterozygous • TTGG, TTGg, TTgg, TtGG, TtGg, Ttgg, ttGG, ttGg, ttgg? • Most important step! 2. Segregate the alleles in each set of genes for each parent • Show meiosis creating the haploid cells
Heterozygous Tall, Heterozygous Green plantcrossed with Heterozygous Tall, Heterozygous Green plant TtGg x TtGg TG Tg tG tg TG Tg tG tg x
TtGg x TtGg Step 3 – Set up and complete the Punnett Square TG Tg tg tG TG T T G G T T G T G G T G t t g g Tg T T T G T T T G t t g g g g g g tG T G G T G G G G t t t t t t g g tg T G T G t t t t t t g g g g g g
Phenotypic Ratio 9 Tall/Green: 3 Tall/yellow: 3 Short/Green: 1 Short /Yellow: 9:3:3:1 Phenotypic Ratio:
Examples for you to work out! • Cross a heterozygous tall, yellow plant with a homozygous tall, heterozygous green plant. • Cross a heterozygous tall, yellow plant with a short, heterozygous green plant.